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| Title | In-cell structures visualize human pre-ribosome assembly in the nucleolus. |
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| Journal, issue, pages | Science, Page eaej0483, Year 2026 |
| Publish date | Sep 24, 2026 |
 Authors | Xiaohan Zhao / Yuki Hayashi / Herman K H Fung / Sara Cuylen-Haering / Julia Mahamid / Christoph W Müller /  |
| PubMed Abstract | In eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly ...In eukaryotes, ribosome biogenesis initiates within the nucleolus, a hallmark multilayered compartment of the nucleus. Structures of pre-ribosomes have been characterized ex situ, but their assembly pathways within human nucleoli have not been described. Here, we used cryogenic correlative light and electron tomography to visualize molecular landscapes within HeLa cell nucleoli and obtained in-cell structural snapshots of both ribosomal subunit precursors, the SSU processome and the pre-60S. These recapitulate major states resolved previously ex situ and reveal additional critical interaction partners, including the RNA exosome, rixosome, and nuclear export receptor CRM1-RanGTP. We further show how pre-ribosome assembly landscapes are altered upon RNA polymerase I inhibition. Our study combines molecular structures with cellular context to elucidate the spatiotemporal assembly pathway of the human ribosome. |
 External links | Science / PubMed:42784657 |
| Methods | EM (subtomogram averaging) |
| Resolution | 6.9 - 19.4 Å |
| Structure data |  EMDB-58993: In-cell structure of the human SSU processome state A'  EMDB-59048: In-cell structure of the human SSU processome state preA1-exo  EMDB-59068: In-cell structure of the human pre-60S state A  EMDB-59069: In-cell structure of the human pre-60S state B  EMDB-59070: In-cell structure of the human pre-60S state C  EMDB-59071: In-cell structure of the human pre-60S state D  EMDB-59072: In-cell structure of the human pre-60S state E  EMDB-59073: In-cell structure of the human pre-60S state F  EMDB-59074: In-cell structure of the human pre-60S state G  EMDB-59075: In-cell structure of the human pre-60S state G*  EMDB-59076: In-cell structure of the human SSU processome state A  EMDB-59077: In-cell structure of the human SSU processome state preA1  EMDB-59078: In-cell structure of the human SSU processome state postA1-exo  EMDB-59079: In-cell structure of the human SSU processome state postA1  EMDB-59080: In-cell structure of the human pre-60S state Ipre  EMDB-59081: In-cell structure of the human pre-60S state Ipost  EMDB-59082: In-cell structure of the human pre-60S state J  EMDB-59083: In-cell structure of the human pre-60S state K  EMDB-59084: In-cell structure of the human pre-60S state KCRM1  EMDB-59085: In-cell structure of the human pre-60S state L  EMDB-59086: In-cell structure of the human SSU processome consensus map  EMDB-59087: In-cell structure of the human Pre-60S consensus map  EMDB-59088: In-cell structure of the human pre-60S state H |
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Homo sapiens (human)