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- EMDB-59077: In-cell structure of the human SSU processome state preA1 -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-59077
TitleIn-cell structure of the human SSU processome state preA1
Map dataIn-cell structure of human SSU processome state preA1
Sample
  • Cell: SSU processome in native HeLa cells
KeywordsIn-cell / SSU processome / human / RIBOSOME
Biological speciesHomo sapiens (human)
Methodsubtomogram averaging / cryo EM / Resolution: 9.3 Å
AuthorsZhao X / Mahamid J / Mueller CW
Funding support Germany, 1 items
OrganizationGrant numberCountry
Other government Germany
CitationJournal: To Be Published
Title: In-cell structure of the human SSU processome state preA1
Authors: Zhao X / Mahamid J / Mueller CW
History
DepositionJul 20, 2026-
Header (metadata) releaseSep 23, 2026-
Map releaseSep 23, 2026-
UpdateSep 23, 2026-
Current statusSep 23, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_59077.png

Downloads & links

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Map

FileDownload / File: emd_59077.map.gz / Format: CCP4 / Size: 95 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationIn-cell structure of human SSU processome state preA1
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
3.43 Å/pix.
x 292 pix.
= 1000.1 Å		3.43 Å/pix.
x 292 pix.
= 1000.1 Å		3.43 Å/pix.
x 292 pix.
= 1000.1 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 3.425 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.01
Minimum - Maximum-0.0062413695 - 0.02788622
Average (Standard dev.)-0.00003302827 (±0.0010128882)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions292292292
Spacing292292292
CellA=B=C: 1000.1 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_59077_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: In-cell structure of human SSU processome state preA1 halfmap1

Fileemd_59077_half_map_1.map
AnnotationIn-cell structure of human SSU processome state preA1 halfmap1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: In-cell structure of human SSU processome state preA1 halfmap2

Fileemd_59077_half_map_2.map
AnnotationIn-cell structure of human SSU processome state preA1 halfmap2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : SSU processome in native HeLa cells

EntireName: SSU processome in native HeLa cells
Components
  • Cell: SSU processome in native HeLa cells

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Supramolecule #1: SSU processome in native HeLa cells

SupramoleculeName: SSU processome in native HeLa cells / type: cell / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7.4
GridModel: Quantifoil R1.2/20 / Material: GOLD / Mesh: 200 / Support film - Material: SILICON DIOXIDE / Pretreatment - Type: PLASMA CLEANING
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 3.41 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 7.0 µm / Nominal defocus min: 3.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 9.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: Warp / Number subtomograms used: 12345
ExtractionNumber tomograms: 305 / Number images used: 275000 / Software - Name: Warp
CTF correctionSoftware - Name: Warp
Details: Tilt series were processed in Warp. Subtomograms were classified and initially refined in RELION. Final multi-particle refinement and reconstruction were performed using M (Warp/M).
Type: PHASE FLIPPING ONLY
Final 3D classificationSoftware - Name: RELION (ver. 4.0.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: Warp
Details: Tilt series were processed in Warp. Subtomograms were classified and initially refined in RELION. Final multi-particle refinement and reconstruction were performed using M (Warp/M).
FSC plot (resolution estimation)

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