EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	High-resolution microtubule structures reveal the structural transitions in αβ-tubulin upon GTP hydrolysis.
ジャーナル・号・ページ	Cell, Vol. 157, Issue 5, Page 1117-1129, Year 2014
掲載日	2014年5月22日
著者	Gregory M Alushin / Gabriel C Lander / Elizabeth H Kellogg / Rui Zhang / David Baker / Eva Nogales /
PubMed 要旨	Dynamic instability, the stochastic switching between growth and shrinkage, is essential for microtubule function. This behavior is driven by GTP hydrolysis in the microtubule lattice and is ...Dynamic instability, the stochastic switching between growth and shrinkage, is essential for microtubule function. This behavior is driven by GTP hydrolysis in the microtubule lattice and is inhibited by anticancer agents like Taxol. We provide insight into the mechanism of dynamic instability, based on high-resolution cryo-EM structures (4.7-5.6 Å) of dynamic microtubules and microtubules stabilized by GMPCPP or Taxol. We infer that hydrolysis leads to a compaction around the E-site nucleotide at longitudinal interfaces, as well as movement of the α-tubulin intermediate domain and H7 helix. Displacement of the C-terminal helices in both α- and β-tubulin subunits suggests an effect on interactions with binding partners that contact this region. Taxol inhibits most of these conformational changes, allosterically inducing a GMPCPP-like state. Lateral interactions are similar in all conditions we examined, suggesting that microtubule lattice stability is primarily modulated at longitudinal interfaces.
リンク	Cell / PubMed:24855948 / PubMed Central
手法	EM (単粒子)
解像度	4.7 - 6.6 Å
構造データ	5895 3j6e EMDB-5895: Cryo EM density of microtubules stabilized by GMPCPP PDB-3j6e: Energy minimized average structure of Microtubules stabilized by GmpCpp 手法: EM (単粒子) / 解像度: 4.7 Å 5896 3j6f EMDB-5896: Cryo EM density of GDP-bound dynamic microtubules PDB-3j6f: Minimized average structure of GDP-bound dynamic microtubules 手法: EM (単粒子) / 解像度: 4.9 Å 5897 3j6g EMDB-5897: Cryo EM density of microtubules stabilized by taxol PDB-3j6g: Minimized average structure of microtubules stabilized by taxol 手法: EM (単粒子) / 解像度: 5.5 Å EMDB-5898: Cryo EM density of microtubules stabilized by GMPCPP, without kinesin 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-5899: Cryo EM density of microtubules bound to GDP, no kinesin 手法: EM (単粒子) / 解像度: 6.3 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMPCPP / GMP-CPP, エネルギー貯蔵分子類似体YM ChemComp-HOH: WATER / 水 ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-TA1: TAXOL / パクリタキセル / 薬剤, 化学療法薬YM / パクリタキセル
由来	sus scrofa (ブタ) Homo sapiens (ヒト)
キーワード	STRUCTURAL PROTEIN (タンパク質) / microtubule (微小管) / GmpCpp / GDP / dynamic / taxol (パクリタキセル)