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TitleCryo-EM near-atomic structure of a dsRNA fungal virus shows ancient structural motifs preserved in the dsRNA viral lineage.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 111, Issue 21, Page 7641-7646, Year 2014
Publish dateMay 27, 2014
AuthorsDaniel Luque / Josué Gómez-Blanco / Damiá Garriga / Axel F Brilot / José M González / Wendy M Havens / José L Carrascosa / Benes L Trus / Nuria Verdaguer / Said A Ghabrial / José R Castón /
PubMed AbstractViruses evolve so rapidly that sequence-based comparison is not suitable for detecting relatedness among distant viruses. Structure-based comparisons suggest that evolution led to a small number of ...Viruses evolve so rapidly that sequence-based comparison is not suitable for detecting relatedness among distant viruses. Structure-based comparisons suggest that evolution led to a small number of viral classes or lineages that can be grouped by capsid protein (CP) folds. Here, we report that the CP structure of the fungal dsRNA Penicillium chrysogenum virus (PcV) shows the progenitor fold of the dsRNA virus lineage and suggests a relationship between lineages. Cryo-EM structure at near-atomic resolution showed that the 982-aa PcV CP is formed by a repeated α-helical core, indicative of gene duplication despite lack of sequence similarity between the two halves. Superimposition of secondary structure elements identified a single "hotspot" at which variation is introduced by insertion of peptide segments. Structural comparison of PcV and other distantly related dsRNA viruses detected preferential insertion sites at which the complexity of the conserved α-helical core, made up of ancestral structural motifs that have acted as a skeleton, might have increased, leading to evolution of the highly varied current structures. Analyses of structural motifs only apparent after systematic structural comparisons indicated that the hallmark fold preserved in the dsRNA virus lineage shares a long (spinal) α-helix tangential to the capsid surface with the head-tailed phage and herpesvirus viral lineage.
External linksProc Natl Acad Sci U S A / PubMed:24821769 / PubMed Central
MethodsEM (single particle)
Resolution4.1 Å
Structure data

5600

3j3i

EMDB-5600: Penicillium Chrysogenum Virus (PcV) capsid structure
PDB-3j3i: Penicillium chrysogenum virus (PcV) capsid structure
Method: EM (single particle) / Resolution: 4.1 Å

Source
  • penicillium chrysogenum virus (isolate caston)
KeywordsVIRUS / double-stranded RNA / fungal virus / viral structure / T=1 virus / duplicated helical fold

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