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-Structure paper
タイトル | Cryo-EM near-atomic structure of a dsRNA fungal virus shows ancient structural motifs preserved in the dsRNA viral lineage. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 111, Issue 21, Page 7641-7646, Year 2014 |
掲載日 | 2014年5月27日 |
![]() | Daniel Luque / Josué Gómez-Blanco / Damiá Garriga / Axel F Brilot / José M González / Wendy M Havens / José L Carrascosa / Benes L Trus / Nuria Verdaguer / Said A Ghabrial / José R Castón / ![]() ![]() |
PubMed 要旨 | Viruses evolve so rapidly that sequence-based comparison is not suitable for detecting relatedness among distant viruses. Structure-based comparisons suggest that evolution led to a small number of ...Viruses evolve so rapidly that sequence-based comparison is not suitable for detecting relatedness among distant viruses. Structure-based comparisons suggest that evolution led to a small number of viral classes or lineages that can be grouped by capsid protein (CP) folds. Here, we report that the CP structure of the fungal dsRNA Penicillium chrysogenum virus (PcV) shows the progenitor fold of the dsRNA virus lineage and suggests a relationship between lineages. Cryo-EM structure at near-atomic resolution showed that the 982-aa PcV CP is formed by a repeated α-helical core, indicative of gene duplication despite lack of sequence similarity between the two halves. Superimposition of secondary structure elements identified a single "hotspot" at which variation is introduced by insertion of peptide segments. Structural comparison of PcV and other distantly related dsRNA viruses detected preferential insertion sites at which the complexity of the conserved α-helical core, made up of ancestral structural motifs that have acted as a skeleton, might have increased, leading to evolution of the highly varied current structures. Analyses of structural motifs only apparent after systematic structural comparisons indicated that the hallmark fold preserved in the dsRNA virus lineage shares a long (spinal) α-helix tangential to the capsid surface with the head-tailed phage and herpesvirus viral lineage. |
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手法 | EM (単粒子) |
解像度 | 4.1 Å |
構造データ | |
由来 |
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![]() | VIRUS / double-stranded RNA / fungal virus / viral structure / T=1 virus / duplicated helical fold |