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TitleStructural basis of human γTuRC closure during CM1-activated microtubule nucleation.
Journal, issue, pagesNat Commun, Vol. 17, Issue 1, Year 2026
Publish dateMar 26, 2026
AuthorsMarina Serna / Cláudia Brito / Silvia Speroni / Fabian Zimmermann / Andrés Lopez-Perrote / Maria Gili / Cristina Lacasa / Jens Lüders / Thomas Surrey / Oscar Llorca /
PubMed AbstractMicrotubule nucleation by the γ-tubulin ring complex (γTuRC) is spatiotemporally regulated and in higher eukaryotes is thought to involve a transition from an inactive open to an active closed ...Microtubule nucleation by the γ-tubulin ring complex (γTuRC) is spatiotemporally regulated and in higher eukaryotes is thought to involve a transition from an inactive open to an active closed conformation that matches the microtubule geometry. However, γTuRC activators only promote a partially closed conformation, raising the question of whether complete closure is required for activation. Combining in vitro nucleation assays and cryo-EM, we find that centrosomin motif 1 (CM1), a conserved element of several γTuRC regulators, potently accelerates human γTuRC-mediated microtubule nucleation by facilitating complete closure of γTuRC as the nascent microtubule assembles. A 3.7 Å cryo-EM structure identifies the γTuRC latch and several interactions involved in conformational closure. Notably, the distinct subunits that keep γTuRC open and inactive in higher eukaryotes also participate in its closure and activation. This work provides additional insight into the logic of the human γTuRC architecture and its activation by CM1.
External linksNat Commun / PubMed:41888131 / PubMed Central
MethodsEM (single particle)
Resolution3.67 - 7.62 Å
Structure data

55043

9smx

EMDB-55043, PDB-9smx:
CM1-activated gTuRC in complex with nascent alpha-E254D mutant microtubules
Method: EM (single particle) / Resolution: 3.67 Å

EMDB-55044: CM1-activated gTuRC in complex with nascent wildtype microtubules
Method: EM (single particle) / Resolution: 7.62 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsSTRUCTURAL PROTEIN / Microtubule / cytoskeleton / g-tubulin ring complex / tubulin / CDK5RAP2 / CM1 / nucleation

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