Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex.
Journal, issue, pages	J Cell Biol, Vol. 225, Issue 5, Year 2026
Publish date	May 4, 2026
Authors	Noah N Turner / Ziguo Zhang / Jing Yang / Kyle W Muir / Stephen H McLaughlin / Tomos Morgan / David Barford /
PubMed Abstract	During mitosis and meiosis, kinetochores mediate interactions between chromosomes and spindle microtubules. Kinetochores are multi-megadalton protein complexes essential for chromosome segregation; ...During mitosis and meiosis, kinetochores mediate interactions between chromosomes and spindle microtubules. Kinetochores are multi-megadalton protein complexes essential for chromosome segregation; however, recent structural, functional, and evolutionary studies have revealed divergent mechanisms of kinetochore assembly. Here, we use cryo-EM to understand the structural mechanisms by which the budding yeast microtubule-binding outer kinetochore KMN complex assembles, and how its interactions with the centromere-binding inner kinetochore are regulated. The KMN complex comprises three subcomplexes: Knl1c, Mis12cMtw1c, and Ndc80c. We show how C-terminal motifs of the Mis12cMtw1c subunits Dsn1, Mis12Mtw1, and Nnf1 bind Knl1c and Ndc80c. At the opposite end of the Mis12cMtw1c stalk, an N-terminal auto-inhibitory segment of Dsn1 (Dsn1AI) folds into two α-helices that engage the Mis12cMtw1c head 1 domain, thereby occluding binding sites for the inner kinetochore subunits CENP-CMif2 and CENP-UAme1, reducing their affinity for Mis12cMtw1. Our structure reveals how Aurora BIpl1 phosphorylation of Dsn1AI would release this auto-inhibition to substantially strengthen preexisting connections between the inner and outer kinetochore.
External links	J Cell Biol / PubMed:41956986 / PubMed Central
Methods	EM (single particle)
Resolution	4.8 - 7.2 Å
Structure data	EMDB-54576: Consensus cryo-EM map of the Saccharomyces cerevisiae KMN junction complex lacking the Mis12c(Mtw1c) head 2 domain Method: EM (single particle) / Resolution: 6.0 Å EMDB-54577: Mutlbody refinement cryo-EM density map of the base of the Saccharomyces cerevisiae KMN junction complex Method: EM (single particle) / Resolution: 4.9 Å EMDB-54578: Multibody refinement cryo-EM density map of the apex of the Saccharomyces cerevisiae KMN junction complex Method: EM (single particle) / Resolution: 4.8 Å 54579 9s4q EMDB-54579: Composite cryo-EM density map of the Saccharomyces cerevisiae KMN junction complex lacking the Mis12c(Mtw1c) head 2 domain PDB-9s4q: Cryo-EM structure of the Saccharomyces cerevisiae KMN junction complex lacking the Mis12c(Mtw1c) head 2 domain Method: EM (single particle) / Resolution: 4.9 Å 54586 9s53 EMDB-54586: Multibody refinement cryo-EM density map of the base of the Saccharomyces cerevisiae KMN junction complex with Mis12c(Mtw1c) head 2 domain resolved PDB-9s53: Cryo-EM structure of the base of the Saccharomyces cerevisiae KMN junction complex containing the Mis12c(Mtw1c) head 2 domain Method: EM (single particle) / Resolution: 6.5 Å 54602 9s5n EMDB-54602, PDB-9s5n: Cryo-EM structure of the Saccharomyces cerevisiae KMN junction complex containing the Mis12c(Mtw1c) head 2 domain Method: EM (single particle) / Resolution: 7.2 Å
Source	saccharomyces cerevisiae (brewer's yeast) saccharomyces cerevisiae s288c (yeast)
Keywords	CELL CYCLE / Kinetochore / chromosome segregation / mitosis