EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A molecular basis for stoichiometric enzyme encapsulation in the vitamin B2 biosynthesis compartment.
ジャーナル・号・ページ	Nat Commun, Year 2026
掲載日	2026年5月16日
著者	Lukasz Koziej / Jedrzej Pankowski / Monika Stefanska / Daniel Jankowski / Agnieszka Gawin / V Vishal Malolan / Juha T Huiskonen / Takahiro Kosugi / Yusuke Azuma /
PubMed 要旨	Encapsulating metabolic enzymes within protein cages enhances catalytic efficiency through substrate channeling. The vitamin B2 biosynthesis system, in which a dodecahedral lumazine synthase (LS) ...Encapsulating metabolic enzymes within protein cages enhances catalytic efficiency through substrate channeling. The vitamin B2 biosynthesis system, in which a dodecahedral lumazine synthase (LS) cage encapsulates a homotrimeric riboflavin synthase (RS), exemplifies this strategy, yet the molecular basis for this stoichiometric enzyme encapsulation has remained elusive. Here, cryogenic electron microscopy structures reveal a hierarchical assembly mechanism that ensures the defined host-guest ratio. RS C-terminal cage-localization signal peptides anchor at LS pentamer-pentamer interfaces early during assembly, stabilizing open intermediates that, together with delayed later-stage cage closure, extend the loading window until guest incorporation is complete. RS spatial occupancy avoids overloading, while a molecular lock upon final closure prevents disassembly. The elucidated anchoring mechanism enabled structure-based phylogenetic analysis across diverse organisms, suggesting multiple independent evolutionary origins of this modular encapsulation strategy. This naturally occurring architecture provides design principles for engineering synthetic catalytic compartments with programmable stoichiometric control.
リンク	Nat Commun / PubMed:42143052
手法	EM (単粒子)
解像度	1.71 - 2.78 Å
構造データ	54381 9ryi EMDB-54381, PDB-9ryi: Aquifex aeolicus lumazine synthase 12-pentamer cage in complex with I-symmetry-masked riboflavin synthase 手法: EM (単粒子) / 解像度: 1.71 Å 54382 9ryj EMDB-54382, PDB-9ryj: Aquifex aeolicus lumazine synthase 11-pentamer cage in complex with C5-symmetrized riboflavin synthase C-termini 手法: EM (単粒子) / 解像度: 1.99 Å 54383 9ryk EMDB-54383, PDB-9ryk: Aquifex aeolicus lumazine synthase 11-pentamer cage in complex with riboflavin synthase trimer 手法: EM (単粒子) / 解像度: 2.6 Å 54385 9rym EMDB-54385, PDB-9rym: Aquifex aeolicus lumazine synthase 10-pentamer cage in complex with riboflavin synthase trimer 手法: EM (単粒子) / 解像度: 2.78 Å 54386 9ryn EMDB-54386, PDB-9ryn: Aquifex aeolicus lumazine synthase 12-pentamer cage 手法: EM (単粒子) / 解像度: 1.71 Å 54387 9ryo EMDB-54387, PDB-9ryo: Aquifex aeolicus lumazine synthase 11-pentamer cage 手法: EM (単粒子) / 解像度: 2.09 Å 54388 9ryp EMDB-54388, PDB-9ryp: Aquifex aeolicus lumazine synthase R29A mutant 12-pentamer cage 手法: EM (単粒子) / 解像度: 1.71 Å 54389 9ryq EMDB-54389, PDB-9ryq: Aquifex aeolicus lumazine synthase R29A mutant 11-pentamer cage 手法: EM (単粒子) / 解像度: 2.08 Å 54392 9ryu EMDB-54392, PDB-9ryu: Aquifex aeolicus lumazine synthase L121A mutant 12-pentamer cage 手法: EM (単粒子) / 解像度: 1.71 Å 54393 9ryv EMDB-54393, PDB-9ryv: Aquifex aeolicus lumazine synthase L121A mutant 11-pentamer cage 手法: EM (単粒子) / 解像度: 2.49 Å 54394 9ryw EMDB-54394, PDB-9ryw: Aquifex aeolicus lumazine synthase I125A mutant 12-pentamer cage 手法: EM (単粒子) / 解像度: 1.71 Å 54395 9ryx EMDB-54395, PDB-9ryx: Aquifex aeolicus lumazine synthase I125A mutant 11-pentamer cage 手法: EM (単粒子) / 解像度: 2.31 Å
化合物	ChemComp-PO4: PHOSPHATE ION ChemComp-HOH: WATER
由来	aquifex aeolicus vf5 (バクテリア)
キーワード	BIOSYNTHETIC PROTEIN / protein cage / protein engineering / self-assembly / geometry / pentamer / encapsulation