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TitleStructural characterisation of chromatin remodelling intermediates supports linker DNA dependent product inhibition as a mechanism for nucleosome spacing.
Journal, issue, pagesElife, Vol. 14, Year 2025
Publish dateDec 24, 2025
AuthorsAmanda L Hughes / Ramasubramanian Sundaramoorthy / Tom Owen-Hughes /
PubMed AbstractPreviously we showed that Chd1 chromatin remodelling enzyme associates with nucleosomes oriented towards the longer linker (Sundaramoorthy et al., 2018) (1). Here we report a series of structures of ...Previously we showed that Chd1 chromatin remodelling enzyme associates with nucleosomes oriented towards the longer linker (Sundaramoorthy et al., 2018) (1). Here we report a series of structures of Chd1 bound to nucleosomes during ongoing ATP-dependent repositioning. Combining these with biochemical experiments and existing literature we propose a model in which Chd1 first associates oriented to sample putative entry DNA. In an ATP-dependent reaction, the enzyme then redistributes to the opposite side of the nucleosome, where it subsequently adopts a conformation productive for DNA translocation. Once this active complex extends nascent exit linker to approximately 15bp, it is sensed by the Chd1 DNA binding domain resulting in conversion to a product inhibited state. These observations provide a mechanistic basis for the action of a molecular ruler element in nucleosome spacing.
External linksElife / PubMed:41439750
MethodsEM (single particle)
Resolution3.8 - 4.2 Å
Structure data

53590

9r5k

EMDB-53590, PDB-9r5k:
Structural characterisation of chromatin remodelling intermediates supports linker DNA dependent product inhibition as a mechanism for nucleosome spacing.
Method: EM (single particle) / Resolution: 4.2 Å

53595

9r5s

EMDB-53595, PDB-9r5s:
Structural characterisation of chromatin remodelling intermediates supports linker DNA dependent product inhibition as a mechanism for nucleosome spacing.
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)
  • xenopus laevis (African clawed frog)
KeywordsGENE REGULATION / Nucleosome / Remodelling enzyme

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