EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the Methanosarcina mazei Mtr complex bound to the oxygen-stress responsive small protein MtrI.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Page 133, Year 2025
掲載日	2025年12月23日
著者	Tristan Reif-Trauttmansdorff / Eva Herdering / Stefan Bohn / Tomas Pascoa / Jörg Kahnt / Erik Zimmer / Anuj Kumar / Ruth A Schmitz / Jan M Schuller /
PubMed 要旨	Methanogenic archaea emit ~1 Gt of methane annually, impacting global carbon cycling and climate. Central to their energy metabolism is a membrane-bound, sodium-translocating methyltransferase ...Methanogenic archaea emit ~1 Gt of methane annually, impacting global carbon cycling and climate. Central to their energy metabolism is a membrane-bound, sodium-translocating methyltransferase complex: the N⁵-tetrahydromethanopterin:CoM-S-methyltransferase (Mtr). It couples methyl transfer between two methanogen-specific cofactors with sodium ion transport across the membrane, forming the only energy-conserving step in hydrogenotrophic methanogenesis. Here, we present a 2.1 Å single-particle cryo-EM structure of the Mtr complex from Methanosarcina mazei. The structure reveals the organization of all catalytic subunits, embedded archaeal lipids and the sodium-binding site. Most strikingly, we discover MtrI, a previously unannotated small open-reading frame encoded protein ( < 100 aa) found within the order of Methanosarcinales that binds both the top of the sodium-channel and cytosolic domain of MtrA via its cobamide cofactor in response to oxygen exposure. This interaction likely prevents sodium leakage and stabilizes the complex under oxidative conditions, revealing an unexpected regulatory mechanism in methanogen energy conservation.
リンク	Nat Commun / PubMed:41436738 / PubMed Central
手法	EM (単粒子)
解像度	2.1 - 3.2 Å
構造データ	53358 9qtp EMDB-53358, PDB-9qtp: Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder 手法: EM (単粒子) / 解像度: 3.2 Å 53359 9qtq EMDB-53359, PDB-9qtq: Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder 手法: EM (単粒子) / 解像度: 2.1 Å 53360 9qtr EMDB-53360, PDB-9qtr: Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder 手法: EM (単粒子) / 解像度: 2.5 Å 53361 9qts EMDB-53361, PDB-9qts: Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder 手法: EM (単粒子) / 解像度: 2.1 Å
化合物	PDB-1jap: COMPLEX OF PRO-LEU-GLY-HYDROXYLAMINE WITH THE CATALYTIC DOMAIN OF MATRIX METALLO PROTEINASE-8 (MET80 FORM) ChemComp-L1P: 3-PHOSPHORYL-[1,2-DI-PHYTANYL]GLYCEROL / りん酸(2S)-2,3-ビス[[(3R,7R,11R)-3,7,11,15-テトラメチルヘキサデシル]オキシ]プロピル PDB-1jao: COMPLEX OF 3-MERCAPTO-2-BENZYLPROPANOYL-ALA-GLY-NH2 WITH THE CATALYTIC DOMAIN OF MATRIX METALLO PROTEINASE-8 (MET80 FORM) ChemComp-NA: Unknown entry / ナトリウムカチオン PDB-1jan: COMPLEX OF PRO-LEU-GLY-HYDROXYLAMINE WITH THE CATALYTIC DOMAIN OF MATRIX METALLO PROTEINASE-8 (PHE79 FORM) ChemComp-HOH: WATER ChemComp-B13: 5-HYDROXYBENZIMIDAZOLYLCOB(III)AMIDE
由来	methanosarcina mazei go1 (古細菌)
キーワード	MEMBRANE PROTEIN / Sodium-pumping / membrane-bound / methyltransferase complex / methanogen / methanogenic / methylotrophic / archaeon / archaea / methanosarcina / methanosarcina mazei / Vitamin B12 / corrinoid / cobalt / 5-hydroxybenzimidazole / small protein / oxygen-sensitive