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-Structure paper
| タイトル | A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system. |
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| ジャーナル・号・ページ | Sci Adv, Vol. 11, Issue 47, Page eady9587, Year 2025 |
| 掲載日 | 2025年11月21日 |
著者 | Gabriel U Oka / Nathanaël Benoit / Axel Siroy / Francesca Gubellini / Esther Marza / Rémi Fronzes / ![]() |
| PubMed 要旨 | Type VII secretion systems (T7SS) are protein translocation machines crucial for virulence and bacterial competition in Gram-positive bacteria. Despite their importance, the structural basis for ...Type VII secretion systems (T7SS) are protein translocation machines crucial for virulence and bacterial competition in Gram-positive bacteria. Despite their importance, the structural basis for assembly of type VIIb secretion systems (T7SSb), a widely distributed variant in Firmicutes, remains poorly understood. We present the cryo-electron microscopy structure of the T7SSb core complex from , revealing how the ubiquitin-like protein YukD, coordinates assembly of the secretion machinery. YukD interacts extensively with the central channel component YukB and facilitates its association with the pseudokinase YukC, forming a stable building block for channel assembly. Time-lapse microscopy and competition assays demonstrate that YukD is essential for proper T7SSb complex formation and contact-dependent bacterial killing. Our findings reveal how bacteria have adapted a ubiquitin-like protein as a structural regulator for assembling a large secretion complex. |
リンク | Sci Adv / PubMed:41270170 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.8 - 7.2 Å |
| 構造データ | ![]() EMDB-53202: Focused Cryo-EM Map of the Dimeric Type VIIb Core Unit (T7bCU) with an additional YukB DUF and stalk domain ![]() EMDB-53218: Focused Cryo-EM Map of the Dimeric Type VIIb Core Unit (T7bCU) ![]() EMDB-53219: Focused Cryo-EM Map of the Type VIIb Core Unit (T7bCU) with an Additional YukB DUF and Stalk Domain EMDB-53220, PDB-9qks: |
| 由来 |
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キーワード | PROTEIN TRANSPORT / T7SS / Type VII Secretion System / T7bCU |
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