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TitleA ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system.
Journal, issue, pagesSci Adv, Vol. 11, Issue 47, Page eady9587, Year 2025
Publish dateNov 21, 2025
AuthorsGabriel U Oka / Nathanaël Benoit / Axel Siroy / Francesca Gubellini / Esther Marza / Rémi Fronzes /
PubMed AbstractType VII secretion systems (T7SS) are protein translocation machines crucial for virulence and bacterial competition in Gram-positive bacteria. Despite their importance, the structural basis for ...Type VII secretion systems (T7SS) are protein translocation machines crucial for virulence and bacterial competition in Gram-positive bacteria. Despite their importance, the structural basis for assembly of type VIIb secretion systems (T7SSb), a widely distributed variant in Firmicutes, remains poorly understood. We present the cryo-electron microscopy structure of the T7SSb core complex from , revealing how the ubiquitin-like protein YukD, coordinates assembly of the secretion machinery. YukD interacts extensively with the central channel component YukB and facilitates its association with the pseudokinase YukC, forming a stable building block for channel assembly. Time-lapse microscopy and competition assays demonstrate that YukD is essential for proper T7SSb complex formation and contact-dependent bacterial killing. Our findings reveal how bacteria have adapted a ubiquitin-like protein as a structural regulator for assembling a large secretion complex.
External linksSci Adv / PubMed:41270170 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 7.2 Å
Structure data

EMDB-53202: Focused Cryo-EM Map of the Dimeric Type VIIb Core Unit (T7bCU) with an additional YukB DUF and stalk domain
Method: EM (single particle) / Resolution: 7.2 Å

EMDB-53218: Focused Cryo-EM Map of the Dimeric Type VIIb Core Unit (T7bCU)
Method: EM (single particle) / Resolution: 6.9 Å

EMDB-53219: Focused Cryo-EM Map of the Type VIIb Core Unit (T7bCU) with an Additional YukB DUF and Stalk Domain
Method: EM (single particle) / Resolution: 4.12 Å

53220

9qks

EMDB-53220, PDB-9qks:
B subtilis Type VIIb Core Unit (T7bCU) + DUF
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • bacillus subtilis subsp. subtilis str. 168 (bacteria)
KeywordsPROTEIN TRANSPORT / T7SS / Type VII Secretion System / T7bCU

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