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Structure paper

TitleStructural analysis reveals alternative pathways for assembly of the human mitoribosome small subunit
Journal, issue, pagesTo Be Published
Publish dateOct 22, 2024 (structure data deposition date)
AuthorsSingh V / Shiriaev D / Bilalli L / Khawaja A / Rorbach J
External linksSearch PubMed
MethodsEM (single particle)
Resolution3.0 - 5.96 Å
Structure data

51873

9h51

EMDB-51873, PDB-9h51:
Assembly intermediate of human mitochondrial ribosome small subunit in complex with PUS1, METTL15 and RBFA(IN conformation) (state M1)
Method: EM (single particle) / Resolution: 3.1 Å

51876

9h54

EMDB-51876, PDB-9h54:
Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and partial RBFA (state N2)
Method: EM (single particle) / Resolution: 3.0 Å

51877

9h55

EMDB-51877, PDB-9h55:
Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and TFB1M (state N3)
Method: EM (single particle) / Resolution: 3.4 Å

52857

9igt

EMDB-52857, PDB-9igt:
Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15, RBFA, and mtIF2 (State M2.1)
Method: EM (single particle) / Resolution: 5.96 Å

52858

9igu

EMDB-52858, PDB-9igu:
Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and RBFA (Inward conformation) (State M2)
Method: EM (single particle) / Resolution: 3.64 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-SPM:
SPERMINE

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsRIBOSOME / mitochondria / small subunit / assembly / NOA1 / mitochondrial gene expression / mitoribosome / mitoribosome assembly factors / cryoEM

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