EMDB-52857: Assembly intermediate of human mitochondrial ribosome small subun...

Basic information

Entry

Database: EMDB / ID: EMD-52857

• Title: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15, RBFA, and mtIF2 (State M2.1)
• Map data: Consensus map: METTL15-IF2-RBFA State M2.1/A5.1

Sample

• Cell: Maturation state of mitochondria ribosomal 28S subunit.
  • Protein or peptide: x 32 types
• Protein or peptide: x 1 types
• RNA: x 1 types
• Ligand: x 8 types

• Keywords: mitochondrial gene expression / mitoribosome / mitoribosome assembly factors / cryoEM / RIBOSOME

Function / homology

Function:

rRNA (cytosine-N4-)-methyltransferase activity / mitochondrial translational initiation / translation factor activity, RNA binding / mitochondrial transcription / mitochondrial ribosome assembly / Mitochondrial translation elongation / Mitochondrial translation initiation / Mitochondrial translation termination / negative regulation of mitotic nuclear division / rRNA base methylation / mitochondrial large ribosomal subunit / mitochondrial ribosome / ribosome disassembly / mitochondrial small ribosomal subunit / regulation of translational initiation / mitochondrial translation / apoptotic mitochondrial changes / positive regulation of proteolysis / ribosomal small subunit binding / translation initiation factor activity / Mitochondrial protein degradation / Transferases; Transferring one-carbon groups; Methyltransferases / apoptotic signaling pathway / rRNA processing / cell junction / regulation of translation / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / nuclear membrane / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / tRNA binding / cell population proliferation / mitochondrial inner membrane / rRNA binding / intracellular membrane-bounded organelle / structural constituent of ribosome / ribosome / translation / mitochondrial matrix / protein domain specific binding / GTPase activity / mRNA binding / positive regulation of DNA-templated transcription / GTP binding / nucleolus / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytoplasm / cytosol

Domain/homology:

Ribosomal RNA small subunit methyltransferase H / S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily / MraW methylase family / Putative ribosome-binding factor A, mitochondrial / Ribosome-binding factor A, conserved site / Ribosome-binding factor A signature. / Ribosome-binding factor A / Ribosome-binding factor A domain superfamily / Ribosome-binding factor A / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / : / Elongation factor G domain 2 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / : / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S24, mitochondrial / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / : / Mitochondrial ribosome subunit S24 / Small ribosomal subunit protein uS5m, N-terminal / Small ribosomal subunit protein mS39, PPR / 28S ribosomal protein S25, mitochondrial / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Ribosomal protein L7/L12 dimerisation domain / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Pentatricopeptide repeat / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 superfamily / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Small GTP-binding protein domain / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2

Component:

MRPS21 isoform 1 / 12S rRNA N(4)-cytidine methyltransferase METTL15 / Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS14m / Translation initiation factor IF-2, mitochondrial / Small ribosomal subunit protein mS29 / Large ribosomal subunit protein bL12m / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein mS34 / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein uS9m / Putative ribosome-binding factor A, mitochondrial / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS31 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS39 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein mS40

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 5.96 Å
• Authors: Khawaja A / Singh V / Shiriaev DI / Rorbach J

Funding support

Sweden, 1 items

Organization	Grant number	Country
Knut and Alice Wallenberg Foundation	023405053	Sweden

• Citation: Journal: To Be Published; Title: Structural analysis reveals alternative pathways for assembly of the human mitoribosome small subunit; Authors: Singh V / Shiriaev DI / Bilalli L / Khawaja A / Rorbach J

History

Deposition	Feb 20, 2025	-
Header (metadata) release	Mar 11, 2026	-
Map release	Mar 11, 2026	-
Update	Mar 11, 2026	-
Current status	Mar 11, 2026	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_52857.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Consensus map: METTL15-IF2-RBFA State M2.1/A5.1
• Voxel size: X=Y=Z: 1.01 Å

Density

Contour Level	By AUTHOR: 0.01
Minimum - Maximum	-0.01641004 - 0.049441982
Average (Standard dev.)	-0.000046350655 (±0.0018811328)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 600 600 600 Spacing 600 600 600
Cell	A=B=C: 606.0 Å α=β=γ: 90.0 °

Supplemental data

Additional map: Composite map

• File: emd_52857_additional_1.map
• Annotation: Composite map

Half map: Half map 2: METTL15-IF2-RBFA

• File: emd_52857_half_map_1.map
• Annotation: Half map 2: METTL15-IF2-RBFA

Half map: Half map 1: METTL15-IF2-RBFA

• File: emd_52857_half_map_2.map
• Annotation: Half map 1: METTL15-IF2-RBFA

Sample components

Entire : Maturation state of mitochondria ribosomal 28S subunit.

• Entire: Name: Maturation state of mitochondria ribosomal 28S subunit.

Components

• Cell: Maturation state of mitochondria ribosomal 28S subunit.
  • Protein or peptide: 28S ribosomal protein S2, mitochondrial
  • Protein or peptide: 28S ribosomal protein S24, mitochondrial
  • Protein or peptide: 28S ribosomal protein S5, mitochondrial
  • Protein or peptide: 28S ribosomal protein S6, mitochondrial
  • Protein or peptide: 28S ribosomal protein S7, mitochondrial
  • Protein or peptide: 28S ribosomal protein S9, mitochondrial
  • Protein or peptide: 28S ribosomal protein S10, mitochondrial
  • Protein or peptide: 28S ribosomal protein S11, mitochondrial
  • Protein or peptide: 28S ribosomal protein S12, mitochondrial
  • Protein or peptide: 28S ribosomal protein S14, mitochondrial
  • Protein or peptide: 28S ribosomal protein S15, mitochondrial
  • Protein or peptide: 28S ribosomal protein S16, mitochondrial
  • Protein or peptide: 28S ribosomal protein S17, mitochondrial
  • Protein or peptide: 28S ribosomal protein S18b, mitochondrial
  • Protein or peptide: 28S ribosomal protein S18c, mitochondrial
  • Protein or peptide: MRPS21 isoform 1
  • Protein or peptide: 28S ribosomal protein S22, mitochondrial
  • Protein or peptide: 28S ribosomal protein S23, mitochondrial
  • Protein or peptide: 28S ribosomal protein S25, mitochondrial
  • Protein or peptide: 28S ribosomal protein S26, mitochondrial
  • Protein or peptide: 28S ribosomal protein S27, mitochondrial
  • Protein or peptide: 28S ribosomal protein S28, mitochondrial
  • Protein or peptide: 28S ribosomal protein S29, mitochondrial
  • Protein or peptide: 28S ribosomal protein S31, mitochondrial
  • Protein or peptide: 28S ribosomal protein S33, mitochondrial
  • Protein or peptide: 28S ribosomal protein S34, mitochondrial
  • Protein or peptide: 28S ribosomal protein S35, mitochondrial
  • Protein or peptide: Aurora kinase A-interacting protein
  • Protein or peptide: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
  • Protein or peptide: 12S rRNA N4-methylcytidine (m4C) methyltransferase
  • Protein or peptide: Large ribosomal subunit protein bL12m, short mature form
  • Protein or peptide: Putative ribosome-binding factor A, mitochondrial
• Protein or peptide: Translation initiation factor IF-2, mitochondrial
• RNA: 12S mitochondrial rRNA
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
• Ligand: SPERMINE
• Ligand: water

Supramolecule #1: Maturation state of mitochondria ribosomal 28S subunit.

• Supramolecule: Name: Maturation state of mitochondria ribosomal 28S subunit.; type: cell / ID: 1 / Parent: 0 / Macromolecule list: #1-#30, #33-#34
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: 28S ribosomal protein S2, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S2, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.298238 KDa

Sequence

String:

MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT RYFRGGMLTN ARLLFGPTVR LPDLIIFLHT LNNIFEPHVA VRDAAKMNIP TVGIVDTNCN PCLITYPVPG ND DSPLAVH LYCRLFQTAI TRAKEKRQQV EALYRLQGQK EPGDQGPAHP PGADMSHSL

UniProtKB: Small ribosomal subunit protein uS2m

Macromolecule #2: 28S ribosomal protein S24, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.046164 KDa

Sequence

String:

MAASVCSGLL GPRVLSWSRE LPCAWRALHT SPVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMWGT FPGCLADQLV LKRRGNQLEI CAVVLRQLSP HKYYFLVGYS ETLLSYFYKC PVRLHLQTVP S KVVYKYL

UniProtKB: Small ribosomal subunit protein uS3m

Macromolecule #3: 28S ribosomal protein S5, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 48.094594 KDa

Sequence

String:

MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV EADMIQQREE WDRKKKMKVK RERGWSGNSW GGISLGPPDP GPCGETYEDF DTRILEVRNV FTMTAKEGRK KS IRVLVAV GNGKGAAGFS IGKATDRMDA FRKAKNRAVH HLHYIERYED HTIFHDISLR FKRTHIKMKK QPKGYGLRCH RAI ITICRL IGIKDMYAKV SGSINMLSLT QGLFRGLSRQ ETHQQLADKK GLHVVEIREE CGPLPIVVAS PRGPLRKDPE PEDE VPDVK LDWEDVKTAQ GMKRSVWSNL KRAAT

UniProtKB: Small ribosomal subunit protein uS5m

Macromolecule #4: 28S ribosomal protein S6, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S6, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.250588 KDa

Sequence

String:

MPRYELALIL KAMQRPETAA TLKRTIEALM DRGAIVRDLE NLGERALPYR ISAHSQQHNR GGYFLVDFYA PTAAVESMVE HLSRDIDVI RGNIVKHPLT QELKECEGIV PVPLAEKLYS TKKRKK

UniProtKB: Small ribosomal subunit protein bS6m

Macromolecule #5: 28S ribosomal protein S7, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.186154 KDa

Sequence

String:

MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY QVPVPLPDRR RRFLAMKWMI TECRDKKHQR TLMPEKLSHK LLEAFHNQGP VIKRKHDLHK MAEANRALAH YR WW

UniProtKB: Small ribosomal subunit protein uS7m

Macromolecule #6: 28S ribosomal protein S9, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 45.909859 KDa

Sequence

String:

MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM LLNLEKHQSH LQAKSLLPEK TVTRDVIGSR WLIKEELEEM LVEKLSDLDY MQFIRLLEKL LTSQCGAAEE EF VQRFRRS VTLESKKQLI EPVQYDEQGM AFSKSEGKRK TAKAEAIVYK HGSGRIKVNG IDYQLYFPIT QDREQLMFPF HFV DRLGKH DVTCTVSGGG RSAQAGAIRL AMAKALCSFV TEDEVEWMRQ AGLLTTDPRV RERKKPGQEG ARRKFTWKKR

UniProtKB: Small ribosomal subunit protein uS9m

Macromolecule #7: 28S ribosomal protein S10, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.0335 KDa

Sequence

String:

MAARTAFGAV CRRLWQGLGN FSVNTSKGNT AKNGGLLLST NMKWVQFSNL HVDVPKDLTK PVVTISDEPD ILYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLQSVHIYKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV AMEVTKTQLE QLPEHIKEPI WETLSEEKEE SKS

UniProtKB: Small ribosomal subunit protein uS10m

Macromolecule #8: 28S ribosomal protein S11, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S11, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.663805 KDa

Sequence

String:

MQAVRNAGSR FLRSWTWPQT AGRVVARTPA GTICTGARQL QDAAAKQKVE QNAAPSHTKF SIYPPIPGEE SSLRWAGKKF EEIPIAHIK ASHNNTQIQV VSASNEPLAF ASCGTEGFRN AKKGTGIAAQ TAGIAAAARA KQKGVIHIRV VVKGLGPGRL S AMHGLIMG GLEVISITDN TPIPH(5F0)GCRP RKARKL

UniProtKB: Small ribosomal subunit protein uS11m

Macromolecule #9: 28S ribosomal protein S12, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.200947 KDa

Sequence

String:

MSWSGLLHGL NTSLTCGPAL VPRLWATCSM ATLNQMHRLG PPKRPPRKLG PTEGRPQLKG VVLCTFTRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HTLQEHQIVL VEGGRTQDLP GVKLTVVRGK YDCGHVQKK

UniProtKB: Small ribosomal subunit protein uS12m

Macromolecule #10: 28S ribosomal protein S14, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S14, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.168788 KDa

Sequence

String:

MAAFMLGSLL RTFKQMVPSS ASGQVRSHYV DWRMWRDVKR RKMAYEYADE RLRINSLRKN TILPKILQDV ADEEIAALPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGIQRATW

UniProtKB: Small ribosomal subunit protein uS14m

Macromolecule #11: 28S ribosomal protein S15, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S15, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.903293 KDa

Sequence

String:

MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK NLRNTNYDVF EKICWGLGIE YTFPPLYYRR AHRRFVTKKA LCIRVFQETQ KLKKRRRALK AAAAAQKQAK RR NPDSPAK AIPKTLKDSQ

UniProtKB: Small ribosomal subunit protein uS15m

Macromolecule #12: 28S ribosomal protein S16, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.371899 KDa

Sequence

String:

MVHLTTLLCK AYRGGHLTIR LALGGCTNRP FYRIVAAHNK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNL DRIRHWIGCG AHLSKPMEK LLGLAGFFPL HPMMITNAER LRRKRAREVL LASQKTDAEA TDTEATET

UniProtKB: Small ribosomal subunit protein bS16m

Macromolecule #13: 28S ribosomal protein S17, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.526078 KDa

Sequence

String:

MSVVRSSVHA RWIVGKVIGT KMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLRALP VPRAKHVKHE LAEIVFKVG KVIDPVTGKP CAGTTYLESP LSSETTQLSK NLEELNISSA Q

UniProtKB: Small ribosomal subunit protein uS17m

Macromolecule #14: 28S ribosomal protein S18b, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.440629 KDa

Sequence

String:

MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP QVEPRDLDFS TSHGAVSATP PAPTLVSGDP WYPWYNWKQP PERELSRLRR LYQGHLQEES GPPPESMPKM PP RTPAEAS STGQTGPQSA L

UniProtKB: Small ribosomal subunit protein mS40

Macromolecule #15: 28S ribosomal protein S18c, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S18c, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.876786 KDa

Sequence

String:

MAAVVAVCGG LGRKKLTHLV TAAVSLTHPG THTVLWRRGC SQQVSSNEDL PISMENPYKE PLKKCILCGK HVDYKNVQLL SQFVSPFTG CIYGRHITGL CGKKQKEITK AIKRAQIMGF MPVTYKDPAY LKDPKVCNIR YRE

UniProtKB: Small ribosomal subunit protein bS18m

Macromolecule #16: MRPS21 isoform 1

• Macromolecule: Name: MRPS21 isoform 1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.659494 KDa

Sequence

String:

(ACE)AKHLKFIAR TVMVQEGNVE SAYRTLNRIL TMDGLIEDIK HRRYYEKPCR RRQRESYERC RRIYNMEMAR KINFLM RKN RADPWQGC

UniProtKB: MRPS21 isoform 1

Macromolecule #17: 28S ribosomal protein S22, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S22, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 41.337316 KDa

Sequence

String:

MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ERFIVVREPS GTLRKASWEE RDRMIQVYFP KEGRKILTPI IFKEENLRTM YSQDRHVDVL NLCFAQFEPD ST EYIKVHH KTYEDIDKRG KYDLLRSTRY FGGMVWYFVN NKKIDGLLID QIQRDLIDDA TNLVQLYHVL HPDGQSAQGA KDQ AAEGIN LIKVFAKTEA QKGAYIELTL QTYQEALSRH SAAS

UniProtKB: Small ribosomal subunit protein mS22

Macromolecule #18: 28S ribosomal protein S23, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.805664 KDa

Sequence

String:

MAGSRLETVG SIFSRTRDLV RAGVLKEKPL WFDVYDAFPP LREPVFQRPR VRYGKAKAPI QDIWYHEDRI RAKFYSVYGS GQRAFDLFN PNFKSTCQRF VEKYTELQKL GETDEEKLFV ETGKALLAEG VILRRVGEAR TQHGGSHVSR KSEHLSVRPQ T ALEENETQ KEVPQDQHLE APADQSKGLL PP

UniProtKB: Small ribosomal subunit protein mS23

Macromolecule #19: 28S ribosomal protein S25, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.146428 KDa

Sequence

String:

MPMKGRFPIR RTLQYLSQGN VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQIMMFKNM TPSPFLRFYL DSGEQVLVD VETKSNKEIM EHIRKILGKN EETLREEEEE KKQLSHPANF GPRKYCLREC ICEVEGQVPC PSLVPLPKEM R GKYKAALK ADAQD

UniProtKB: Small ribosomal subunit protein mS25

Macromolecule #20: 28S ribosomal protein S26, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.25982 KDa

Sequence

String:

MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI TRENLEARVE AALDSRKNYN WAITREGLVV RPQRRDS

UniProtKB: Small ribosomal subunit protein mS26

Macromolecule #21: 28S ribosomal protein S27, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S27, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 47.669262 KDa

Sequence

String:

MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM MQEAFEVPST QLLSLYVLFH CLAKKTDFSW EEERNFGASL LLPGLKQKNS VGFSSQLYGY ALLGKVELQQ GL RAVYHNM PLIWKPGYLD RALQVMEKVA ASPEDIKLCR EALDVLGAVL KALTSADGAS EEQSQNDEDN QGSEKLVEQL DIE ETEQSK LPQYLERFKA LHSKLQALGK IESEGLLSLT TQLVKEKLST CEAEDIATYE QNLQQWHLDL VQLIQREQQQ REQA KQEYQ AQKAAKASA

UniProtKB: Small ribosomal subunit protein mS27

Macromolecule #22: 28S ribosomal protein S28, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.878846 KDa

Sequence

String:

MAALCRTRAV AAESHFLRVF LFFRPFRGVG TESGSESGSS NAKEPKTRAG GFASALERHS ELLQKVEPLQ KGSPKNVESF ASMLRHSPL TQMGPAKDKL VIGRIFHIVE NDLYIDFGGK FHCVCRRPEV DGEKYQKGTR VRLRLLDLEL TSRFLGATTD T TVLEANAV LLGIQESKDS RSKEEHHEK

UniProtKB: Small ribosomal subunit protein bS1m

Macromolecule #23: 28S ribosomal protein S29, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S29, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 45.634406 KDa

Sequence

String:

MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL LQSSYNKQRF DQPLEASTWL KNFKTTNERF LNQIKVQEKY VWNKRESTEK GSPLGEVVEQ GITRVRNATD AV GIVLKEL KRQSSLGMFH LLVAVDGINA LWGRTTLKRE DKSPIAPEEL ALVHNLRKMM KNDWHGGAIV SALSQTGSLF KPR KAYLPQ ELLGKEGFDA LDPFIPILVS NYNPKEFESC IQYYLENNWL QHEKAPTEEG KKELLFLSNA NPSLLERHCA YL

UniProtKB: Small ribosomal subunit protein mS29

Macromolecule #24: 28S ribosomal protein S31, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 45.391473 KDa

Sequence

String:

MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF DKQTTKSELL SQLQQHEEES RAQRDAKRPK ISFSNIISDM KVARSATARV RSRPELRIQF DEGYDNYPGQ EK TDDLKKR KNIFTGKRLN IFDMMAVTKE APETDTSPSL WDVEFAKQLA TVNEQPLQNG FEELIQWTKE GKLWEFPINN EAG FDDDGS EFHEHIFLEK HLESFPKQGP IRHFMELVTC GLSKNPYLSV KQKVEHIEWF RNYFNEKKDI LKESNIQFN

UniProtKB: Small ribosomal subunit protein mS31

Macromolecule #25: 28S ribosomal protein S33, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S33, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.657776 KDa

Sequence

String:

MSSLSEYAFR MSRLSARLFG EVTRPTNSKS MKVVKLFSEL PLAKKKETYD WYPNHHTYAE LMQTLRFLGL YRDEHQDFMD EQKRLKKLR GKEKPKKGEG KRAAKRK

UniProtKB: Small ribosomal subunit protein mS33

Macromolecule #26: 28S ribosomal protein S34, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S34, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.695549 KDa

Sequence

String:

MARKKVRPRL IAELARRVRA LREQLNRPRD SQLYAVDYET LTRPFSGRRL PVRAWADVRR ESRLLQLLGR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEHVMYHDW RLVPKHEEEA FTAFTPAPED S LASVPYPP LLRAMIIAER QKNGDTSTEE PMLNVQRIRM EPWDYPAKQE DKGRAKGTPV

UniProtKB: Small ribosomal subunit protein mS34

Macromolecule #27: 28S ribosomal protein S35, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 36.898328 KDa

Sequence

String:

MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA RVVVLRVKLS SLNLDDHAKK KLIKLVGERY CKTTDVLTIK TDRCPLRRQN YDYAVYLLTV LYHESWNTEE WE KSKTEAD MEEYIWENSS SERNILETLL QMKAAEKNME INKEELLGTK EIEEYKKSVV SLKNEEENEN SISQYKESVK RLL NVT

UniProtKB: Small ribosomal subunit protein mS35

Macromolecule #28: Aurora kinase A-interacting protein

• Macromolecule: Name: Aurora kinase A-interacting protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.395326 KDa

Sequence

String:

MLLGRLTSQL LRAVPWAGGR PPWPVSGVLG SRVCGPLYST SPAGPGRAAS LPRKGAQLEL EEMLVPRKMS VSPLESWLTA RCFLPRLDT GTAGTVAPPQ SYQCPPSQIG EGAEQGDEGV ADAPQIQCKN VLKIRRRKMN HHKYRKLVKK TRFLRRKVQE G RLRRKQIK FEKDLRRIWL KAGLKEAPEG WQTPKIYLRG K

UniProtKB: Small ribosomal subunit protein mS38

Macromolecule #29: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

• Macromolecule: Name: Pentatricopeptide repeat domain-containing protein 3, mitochondrial; type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 78.648547 KDa

Sequence

String:

MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA SVDMFDQLLQ AGTTVSLETT NSLLDLLCYY GDQEPSTDYH FQQTGQSEAL EEENDETSRR KAGHQFGVTW RA KNNAERI FSLMPEKNEH SYCTMIRGMV KHRAYEQALN LYTELLNNRL HADVYTFNAL IEATVCAINE KFEEKWSKIL ELL RHMVAQ KVKPNLQTFN TILKCLRRFH VFARSPALQV LREMKAIGIE PSLATYHHII RLFDQPGDPL KRSSFIIYDI MNEL MGKRF SPKDPDDDKF FQSAMSICSS LRDLELAYQV HGLLKTGDNW KFIGPDQHRN FYYSKFFDLI CLMEQIDVTL KWYED LIPS AYFPHSQTMI HLLQALDVAN RLEVIPKIWK DSKEYGHTFR SDLREEILML MARDKHPPEL QVAFADCAAD IKSAYE SQP IRQTAQDWPA TSLNCIAILF LRAGRTQEAW KMLGLFRKHN KIPRSELLNE LMDSAKVSNS PSQAIEVVEL ASAFSLP IC EGLTQRVMSD FAINQEQKEA LSNLTALTSD SDTDSSSDSD SDTSEGK

UniProtKB: Small ribosomal subunit protein mS39

Macromolecule #30: 12S rRNA N4-methylcytidine (m4C) methyltransferase

• Macromolecule: Name: 12S rRNA N4-methylcytidine (m4C) methyltransferase / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 46.18402 KDa

Sequence

String:

MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD QTQAQELHRS QDRDFETMAK LHIPVMVDEV VHCLSPQKG QIFLDMTFGS GGHTKAILQK ESDIVLYALD RDPTAYALAE HLSELYPKQI RAMLGQFSQA EALLMKAGVQ P GTFDGVLM DLGCSSMQLD TPERGFSLRK DGPLDMRMDG GRYPDMPTAA DVVNALDQQA LASILRTYGE EKHAKKIASA IV QARSIYP ITRTQQLASI VAGAFPPSAI YTRKDLLQRS THIATKTFQA LRIFVNNELN ELYTGLKTAQ KFLRPGGRLV ALS FHSLED RIVKRFLLGI SMTERFNLSV RQQVMKTSQL GSDHENTEEV SMRRAPLMWE LIHKKVLSPQ DQDVQDNPRG RSAK LRAAI KL

UniProtKB: 12S rRNA N(4)-cytidine methyltransferase METTL15

Macromolecule #32: Translation initiation factor IF-2, mitochondrial

• Macromolecule: Name: Translation initiation factor IF-2, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 76.873039 KDa

Sequence

String:

MSSAYPVWTA QLCAWPWPTD VLTGAALSQY RLLVTKKEEG PWKSQLSSTK SKKVVEVWIG MTIEELARAM EKNTDYVYEA LLNTDIDID SLEADSHLDE VWIKEVITKA GMKLKWSKLK QDKVRKNKDA VRRPQADPAL LTPRSPVVTI MGHVDHGKTT L LDKFRKTQ VAAVETGGIT QHIGAFLVSL PSGEKITFLD TPGHAAFSAM RARGAQVTDI VVLVVAADDG VMKQTVESIQ HA KDAQVPI ILAVNKCDKA EADPEKVKKE LLAYDVVCED YGGDVQAVPV SALTGDNLMA LAEATVALAE MLELKADPNG PVE GTVIES FTDKGRGLVT TAIIQRGTLR KGSVLVAGKC WAKVRLMFDE NGKTIDEAYP SMPVGITGWR DLPSAGEEIL EVES EPRAR EVVDWRKYEQ EQEKGQEDLK IIEEKRKEHK EAHQKAREKY GHLLWKKRSI LRFLERKEQI PLKPKEKRER DSNVL SVII KGDVDGSVEA ILNIIDTYDA SHECELELVH FGVGDVSAND VNLAETFDGV IYGFNVNAGN VIQQSAAKKG VKIKLH KII YRLVEDLQEE LSSRLPCAVE EHPVGEASIL ATFSVTEGKK KVPVAGCRVQ KGQLEKQKKF KLTRNGHVIW KGSLTSL KH HKDDISIVKT GMDCGLSLDE DNMEFQVGDR IVCYEEKQIQ AKTSWDPGF

UniProtKB: Translation initiation factor IF-2, mitochondrial

Macromolecule #33: Large ribosomal subunit protein bL12m, short mature form

• Macromolecule: Name: Large ribosomal subunit protein bL12m, short mature form; type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.779179 KDa

Sequence

String:

THFTVRLTEA KPVDKVKLIK EIKNYIQGIN LVQAKKLVES LPQEIKANVA KAEAEKIKAA LEAVGGTVVL E

UniProtKB: Large ribosomal subunit protein bL12m

Macromolecule #34: Putative ribosome-binding factor A, mitochondrial

• Macromolecule: Name: Putative ribosome-binding factor A, mitochondrial / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.020297 KDa

Sequence

String:

KNWLKKFASK TKKKVWYESP SLGSHSTYKP SKLEFLMRST SKKTRKEDHA RLRALNGLLY KALTDLLCTP EVSQELYDLN VELSKVSLT PDFSACRAYW KTTLSAEQNA HMEAVLQRSA AHMRHLLMSQ QTLRNVPPIV FVQDKGNAAL AELDQLLAVA D FGPRDERD NFVQNDFRDP DAPQPCGTTE PTTSSSLCGI DHEALNKQIM EYKRRKDKGL GGLVWQGQVA ELTTQMKKGR KR AKPRLEQ DSSLKSYLSG EEVEDDLDLV GAPEYECYAP DTEELEAERG GGRTEDGHSC GASRE

UniProtKB: Putative ribosome-binding factor A, mitochondrial

Macromolecule #31: 12S mitochondrial rRNA

• Macromolecule: Name: 12S mitochondrial rRNA / type: rna / ID: 31 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 306.228312 KDa

Sequence

String:

AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC AGCAGUGAUU AACCUUUAGC AAUAAACGAA AGUUUAACUA AGCUAUACUA ACCCCAGGGU UGGUCAAUUU CG UGCCAGC CACCGCGGUC ACACGAUUAA CCCAAGUCAA UAGAAGCCGG CGUAAAGAGU GUUUUAGAUC ACCCCCUCCC CAA UAAAGC UAAAACUCAC CUGAGUUGUA AAAAACUCCA GUUGACACAA AAUAGACUAC GAAAGUGGCU UUAACAUAUC UGAA CACAC AAUAGCUAAG ACCCAAACUG GGAUUAGAUA CCCCACUAUG CUUAGCCCUA AACCUCAACA GUUAAAUCAA CAAAA CUGC UCGCCAGAAC ACUACGAGCC ACAGCUUAAA ACUCAAAGGA CCUGGCGGUG CUUCAUAUCC CUCUAGAGGA GCCUGU UCU GUAAUCGAUA AACCCCGAUC AACCUCACCA CCUCUUGCUC AGCCUAUAUA CCGCCAUCUU CAGCAAACCC UGAUGAA GG CUACAAAGUA AGCGCAAGUA CCCACGUAAA GACGUUAGGU CAAGGUGUAG CCCAUGAGGU GGCAAGAAAU GGGCUACA U UUUCUACCCC AGAAAACUAC GAUAGCCCUU AUGAAACUUA AGGGUCGAAG GUGGAUUUAG CAGUAAACUG AGAGUAGAG UGCUUAGUUG AACAGGGCCC UGAAGCGCGU ACACACCG(B8T)C (5MC)GUCACCCUC CUCAAGUAUA CUUCAAAGGA CA UUUAACU AAAACCCCUA CGCAUUUAUA UAGAGGAGAC AAGUCGUAAC AUGGUAAGUG UACUGG(MA6)(MA6)AG UGCAC UUGG ACGAAC

Macromolecule #35: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 35 / Number of copies: 12 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #36: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 36 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #37: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 37 / Number of copies: 2 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Macromolecule #38: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 38 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #39: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 39 / Number of copies: 2 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #40: NICOTINAMIDE-ADENINE-DINUCLEOTIDE

• Macromolecule: Name: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / type: ligand / ID: 40 / Number of copies: 1 / Formula: NAD
• Molecular weight: Theoretical: 663.425 Da

Chemical component information

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

Macromolecule #41: SPERMINE

• Macromolecule: Name: SPERMINE / type: ligand / ID: 41 / Number of copies: 1 / Formula: SPM
• Molecular weight: Theoretical: 202.34 Da

Chemical component information

ChemComp-SPM:
SPERMINE

Macromolecule #42: water

• Macromolecule: Name: water / type: ligand / ID: 42 / Number of copies: 8 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.9000000000000001 µm / Nominal defocus min: 0.3 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

7pnx

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 5.96 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 2833
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD