EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of TOM complexes with bound preproteins.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 122, Issue 29, Page e2507279122, Year 2025
掲載日	2025年7月22日
著者	Ahmed-Noor A Agip / Pamela Ornelas / Tzu-Jing Yang / Ermanno Uboldi / Sabine Häder / Melanie A McDowell / Werner Kühlbrandt /
PubMed 要旨	Mitochondria import most of their proteins from the cytoplasm through the TOM complex. Preproteins containing targeting signals are recognized by the TOM receptor subunits and translocated by Tom40 ...Mitochondria import most of their proteins from the cytoplasm through the TOM complex. Preproteins containing targeting signals are recognized by the TOM receptor subunits and translocated by Tom40 across the outer mitochondrial membrane. We present four structures of the preprotein-bound and preprotein-free TOM core and holo complexes from the thermophilic fungus , obtained by single-particle electron cryomicroscopy. Our structures reveal the symmetric arrangement of two copies of the Tom20 receptor subunit in the TOM holo complex. Several different conformations of Tom20 within the TOM holo complex highlight the dynamic nature of the receptor. The structure of preprotein-bound Tom20 provides insight into the early stages of protein translocation.
リンク	Proc Natl Acad Sci U S A / PubMed:40674418 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.8 Å
構造データ	52652 9i6b EMDB-52652, PDB-9i6b: CryoEM structure of the Chaetomium thermophilum TOM core complex at 2.7 angstrom resolution (pALDH treated) 手法: EM (単粒子) / 解像度: 2.7 Å 52658 9i7p EMDB-52658, PDB-9i7p: CryoEM structure of the Chaetomium thermophilum TOM core complex at 3.2 angstrom resolution 手法: EM (単粒子) / 解像度: 3.2 Å 52660 9i7s EMDB-52660, PDB-9i7s: CryoEM structure of the Chaetomium thermophilum TOM holo complex at 3.2 angstrom resolution (pALDH treated) 手法: EM (単粒子) / 解像度: 3.2 Å 52661 9i7t EMDB-52661, PDB-9i7t: CryoEM structure of the Chaetomium thermophilum TOM holo complex at 3.8 angstrom resolution 手法: EM (単粒子) / 解像度: 3.8 Å
化合物	ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-DU0: 2-[2-[(1~{S},2~{S},4~{S},5'~{R},6~{R},7~{S},8~{R},9~{S},12~{S},13~{R},16~{S})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol ChemComp-PLC: DIUNDECYL PHOSPHATIDYL CHOLINE / DLPC / リン脂質YM
由来	thermochaetoides thermophila dsm 1495 (菌類)
キーワード	MEMBRANE PROTEIN / Mitochondria