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TitleStructures of TOM complexes with bound preproteins.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 122, Issue 29, Page e2507279122, Year 2025
Publish dateJul 22, 2025
AuthorsAhmed-Noor A Agip / Pamela Ornelas / Tzu-Jing Yang / Ermanno Uboldi / Sabine Häder / Melanie A McDowell / Werner Kühlbrandt /
PubMed AbstractMitochondria import most of their proteins from the cytoplasm through the TOM complex. Preproteins containing targeting signals are recognized by the TOM receptor subunits and translocated by Tom40 ...Mitochondria import most of their proteins from the cytoplasm through the TOM complex. Preproteins containing targeting signals are recognized by the TOM receptor subunits and translocated by Tom40 across the outer mitochondrial membrane. We present four structures of the preprotein-bound and preprotein-free TOM core and holo complexes from the thermophilic fungus , obtained by single-particle electron cryomicroscopy. Our structures reveal the symmetric arrangement of two copies of the Tom20 receptor subunit in the TOM holo complex. Several different conformations of Tom20 within the TOM holo complex highlight the dynamic nature of the receptor. The structure of preprotein-bound Tom20 provides insight into the early stages of protein translocation.
External linksProc Natl Acad Sci U S A / PubMed:40674418 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 3.8 Å
Structure data

52652

9i6b

EMDB-52652, PDB-9i6b:
CryoEM structure of the Chaetomium thermophilum TOM core complex at 2.7 angstrom resolution (pALDH treated)
Method: EM (single particle) / Resolution: 2.7 Å

52658

9i7p

EMDB-52658, PDB-9i7p:
CryoEM structure of the Chaetomium thermophilum TOM core complex at 3.2 angstrom resolution
Method: EM (single particle) / Resolution: 3.2 Å

52660

9i7s

EMDB-52660, PDB-9i7s:
CryoEM structure of the Chaetomium thermophilum TOM holo complex at 3.2 angstrom resolution (pALDH treated)
Method: EM (single particle) / Resolution: 3.2 Å

52661

9i7t

EMDB-52661, PDB-9i7t:
CryoEM structure of the Chaetomium thermophilum TOM holo complex at 3.8 angstrom resolution
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

ChemComp-DU0:
2-[2-[(1~{S},2~{S},4~{S},5'~{R},6~{R},7~{S},8~{R},9~{S},12~{S},13~{R},16~{S})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol

ChemComp-PLC:
DIUNDECYL PHOSPHATIDYL CHOLINE / phospholipid*YM

Source
  • thermochaetoides thermophila dsm 1495 (fungus)
KeywordsMEMBRANE PROTEIN / Mitochondria

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