Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structurally heterogeneous ribosomes cooperate in protein synthesis in bacterial cells.
Journal, issue, pages	Nat Commun, Vol. 16, Issue 1, Page 2751, Year 2025
Publish date	Mar 20, 2025
Authors	Karla Helena-Bueno / Sophie Kopetschke / Sebastian Filbeck / Lewis I Chan / Sonia Birsan / Arnaud Baslé / Maisie Hudson / Stefan Pfeffer / Chris H Hill / Sergey V Melnikov /
PubMed Abstract	Ribosome heterogeneity is a paradigm in biology, pertaining to the existence of structurally distinct populations of ribosomes within a single organism or cell. This concept suggests that ...Ribosome heterogeneity is a paradigm in biology, pertaining to the existence of structurally distinct populations of ribosomes within a single organism or cell. This concept suggests that structurally distinct pools of ribosomes have different functional properties and may be used to translate specific mRNAs. However, it is unknown to what extent structural heterogeneity reflects genuine functional specialization rather than stochastic variations in ribosome assembly. Here, we address this question by combining cryo-electron microscopy and tomography to observe individual structurally heterogeneous ribosomes in bacterial cells. We show that 70% of ribosomes in Psychrobacter urativorans contain a second copy of the ribosomal protein bS20 at a previously unknown binding site on the large ribosomal subunit. We then determine that this second bS20 copy appears to be functionally neutral. This demonstrates that ribosome heterogeneity does not necessarily lead to functional specialization, even when it involves significant variations such as the presence or absence of a ribosomal protein. Instead, we show that heterogeneous ribosomes can cooperate in general protein synthesis rather than specialize in translating discrete populations of mRNA.
External links	Nat Commun / PubMed:40113756 / PubMed Central
Methods	EM (single particle) / EM (subtomogram averaging) / EM (tomography)
Resolution	3.2 - 8.2 Å
Structure data	52036 9hc4 EMDB-52036, PDB-9hc4: Cryo-EM structure of P. urativorans 70S ribosome with 2 copies of bS20. Method: EM (single particle) / Resolution: 3.2 Å EMDB-52351: subtomogram average of the P. urativorans 70S ribosome Method: EM (subtomogram averaging) / Resolution: 7.8 Å EMDB-52352: subtomogram average of the P. urativorans 70S ribosome with one copy of bS20 Method: EM (subtomogram averaging) / Resolution: 8.2 Å EMDB-52354: subtomogram average of the P. urativorans 70S ribosome with two copies of bS20 Method: EM (subtomogram averaging) / Resolution: 7.8 Å EMDB-52842: Cryo-ET of cryo-FIB milled P. urativorans grown at physiological conditions Method: EM (tomography)
Source	psychrobacter urativorans (bacteria)
Keywords	RIBOSOME / ribosome heterogeneity / functionally specialized ribosomes / polysome