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TitleCryo-EM structure of photosystem II supercomplex from a green microalga with extreme phototolerance.
Journal, issue, pagesNat Commun, Vol. 17, Issue 1, Page 341, Year 2026
Publish dateJan 9, 2026
AuthorsRameez Arshad / Ioannis Skalidis / David Kopečný / Sylva Brabencová / Monika Opatíková / Petr Ilík / Pavel Pospíšil / Farzad Hamdi / Sanja Ćavar Zeljković / Martina Kopečná / Pavel Roudnický / Dušan Lazár / Eduard Elias / Roberta Croce / Panagiotis L Kastritis / Roman Kouřil /
PubMed AbstractPhotosystem II (PSII) is essential for energy conversion during oxygenic photosynthesis in plants and algae. Chlorella ohadii, one of the fastest multiplying green algae, thrives under the harsh ...Photosystem II (PSII) is essential for energy conversion during oxygenic photosynthesis in plants and algae. Chlorella ohadii, one of the fastest multiplying green algae, thrives under the harsh desert sun but lacks the standard PSII photoprotective mechanisms involving LhcSR/PsbS proteins or protein phosphorylation. Here, we present the cryo-EM structure of the PSII supercomplex from C. ohadii at 2.9 Å resolution, which is used to determine whether the exceptional resistance to desert conditions has a structural basis in PSII. The structure reveals a distinct PsbO isoform and additional subunits, PsbR and PsbY, which enhance core complex stability through extensive interactions. Furthermore, the trimeric light-harvesting complexes (LHCII) are bound to the PSII core by specific light-harvesting proteins whose down-regulation in response to high-light conditions implies a reduction in the number of bound LHCII trimers. These structural modifications, together with the high accumulation of specific polyamines in the thylakoid membrane, play a key role in maintaining PSII stability and photoprotection, allowing C. ohadii to survive in extreme conditions.
External linksNat Commun / PubMed:41513636 / PubMed Central
MethodsEM (single particle)
Resolution2.95 Å
Structure data

52056

9hd7

EMDB-52056, PDB-9hd7:
Cryo-EM structure of photosystem II C2S2M2L2 supercomplex from the green alga Chlorella ohadii
Method: EM (single particle) / Resolution: 2.95 Å

Chemicals

ChemComp-CHL:
CHLOROPHYLL B

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE

ChemComp-OEX:
CA-MN4-O5 CLUSTER

ChemComp-PHO:
PHEOPHYTIN A

ChemComp-BCR:
BETA-CAROTENE

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE

ChemComp-LNL:
ALPHA-LINOLENIC ACID

ChemComp-STE:
STEARIC ACID

ChemComp-CL:
Unknown entry

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-FE2:
Unknown entry

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-PAM:
PALMITOLEIC ACID

Source
  • chlorella ohadii (plant)
KeywordsMEMBRANE PROTEIN / supercomplex / photosynthesis / chlorophyll / algae

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