EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Laser flash melting cryo-EM samples to overcome preferred orientation.
ジャーナル・号・ページ	Nat Methods, Vol. 22, Issue 9, Page 1880-1886, Year 2025
掲載日	2025年8月28日
著者	Monique S Straub / Oliver F Harder / Nathan J Mowry / Sarah V Barrass / Jakub Hruby / Marcel Drabbels / Ulrich J Lorenz /
PubMed 要旨	Sample preparation remains a bottleneck for protein structure determination by cryo-electron microscopy. A frequently encountered issue is that proteins adsorb to the air-water interface of the ...Sample preparation remains a bottleneck for protein structure determination by cryo-electron microscopy. A frequently encountered issue is that proteins adsorb to the air-water interface of the sample in a limited number of orientations. This makes it challenging to obtain high-resolution reconstructions, or may even cause projects to fail altogether. We have previously observed that laser flash melting and revitrification of cryo-EM samples reduces preferred orientation for large, symmetric particles. Here we demonstrate that our method can in fact be used to scramble the orientation of proteins of a range of sizes and symmetries. The effect can be enhanced for some proteins by increasing the heating rate during flash melting or by depositing amorphous ice onto the sample prior to revitrification. This also allows us to shed light onto the underlying mechanism. Our experiments establish a set of tools for overcoming preferred orientation that can be easily integrated into existing workflows.
リンク	Nat Methods / PubMed:40877464 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 4.1 Å
構造データ	EMDB-51744: Cryo-EM map conventional T20S proteasome 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-51745: Cryo-EM map of revitrified T20S proteasome 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-51746: Cryo-EM map of deposited and revitrified T20S proteasome 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-51747: Cryo-EM map of conventional 50S ribosomal subunit 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-51748: Cryo-EM map of revitrified 50S ribosomal subunit 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-51749: Cryo-EM map of deposited and revitrified 50S ribosomal subunit 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-51750: Cryo-EM map of conventional 50S ribosomal subunit used as control for shaped pulses 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-51751: Cryo-EM map of shaped pulse revitrified 50S ribosomal subunit 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-51752: Cryo-EM map of conventional HIV-1 envelope protein 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-51753: Cryo-EM map of revitrified HIV-1 envelope protein 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-51754: Cryo-EM map of conventional Hemagglutinin 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-51755: Cryo-EM map of revitrified Hemagglutinin 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-51756: Cryo-EM map of deposited and revitrified Hemagglutinin 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-51757: Cryo-EM map of shaped pulse revitrified Hemagglutinin 手法: EM (単粒子) / 解像度: 3.0 Å
由来	Thermoplasma acidophilum (好酸性) E.coli K12 strain BW25113 (大腸菌) E.coli K12 BW25113 (大腸菌) Human immunodeficiency virus 1 (ヒト免疫不全ウイルス) Influenza A virus (A型インフルエンザウイルス)