Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Passage of the HIV capsid cracks the nuclear pore.
Journal, issue, pages	Cell, Vol. 188, Issue 4, Page 930-943.e21, Year 2025
Publish date	Feb 20, 2025
Authors	Jan Philipp Kreysing / Maziar Heidari / Vojtech Zila / Sergio Cruz-León / Agnieszka Obarska-Kosinska / Vibor Laketa / Lara Rohleder / Sonja Welsch / Jürgen Köfinger / Beata Turoňová / Gerhard Hummer / Hans-Georg Kräusslich / Martin Beck /
PubMed Abstract	Upon infection, human immunodeficiency virus type 1 (HIV-1) releases its cone-shaped capsid into the cytoplasm of infected T cells and macrophages. The capsid enters the nuclear pore complex (NPC), ...Upon infection, human immunodeficiency virus type 1 (HIV-1) releases its cone-shaped capsid into the cytoplasm of infected T cells and macrophages. The capsid enters the nuclear pore complex (NPC), driven by interactions with numerous phenylalanine-glycine (FG)-repeat nucleoporins (FG-Nups). Whether NPCs structurally adapt to capsid passage and whether capsids are modified during passage remains unknown, however. Here, we combined super-resolution and correlative microscopy with cryoelectron tomography and molecular simulations to study the nuclear entry of HIV-1 capsids in primary human macrophages. Our data indicate that cytosolically bound cyclophilin A is stripped off capsids entering the NPC, and the capsid hexagonal lattice remains largely intact inside and beyond the central channel. Strikingly, the NPC scaffold rings frequently crack during capsid passage, consistent with computer simulations indicating the need for NPC widening. The unique cone shape of the HIV-1 capsid facilitates its entry into NPCs and helps to crack their rings.
External links	Cell / PubMed:39826544
Methods	EM (subtomogram averaging)
Resolution	24.5 - 36.7 Å
Structure data	EMDB-51531: HIV-1 CA hexamer from capsids inside virions (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 30.1 Å EMDB-51532: HIV-1 CA hexamer from cytoplasmic capsids (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 27.7 Å EMDB-51533: HIV-1 CA hexamer from capsids inside NPCs (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 33.0 Å EMDB-51534: HIV-1 CA hexamer from nuclear capsids (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 29.7 Å EMDB-51535: HIV-1 CA pentamer from capsids inside virions (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 29.0 Å EMDB-51536: HIV-1 CA pentamer from cytoplasmic capsids (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 29.2 Å EMDB-51626: In situ human cytoplasmic ring subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 24.5 Å EMDB-51627: In situ human inner ring subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 28.5 Å EMDB-51628: In situ human nuclear ring subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 27.9 Å EMDB-51629: In situ human luminal ring subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 28.5 Å EMDB-51630: In situ human basket attachment to nuclear ring of nuclear pore complex (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 33.2 Å EMDB-51631: In situ human asymmetric subunit of nuclear pore complex (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 31.8 Å EMDB-51632: In situ human symmetrized composite map of nuclear pore complex (FIB-lamella data of HIV infected macrophages) Method: EM (subtomogram averaging) / Resolution: 28.0 Å EMDB-52138: In situ human cytoplasmic ring subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages) Method: EM (subtomogram averaging) / Resolution: 27.9 Å EMDB-52139: In situ human inner ring subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages) Method: EM (subtomogram averaging) / Resolution: 30.1 Å EMDB-52140: In situ human nuclear ring subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages) Method: EM (subtomogram averaging) / Resolution: 30.4 Å EMDB-52141: In situ human luminal ring subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages) Method: EM (subtomogram averaging) / Resolution: 29.1 Å EMDB-52142: In situ human basket attachment to nuclear ring of nuclear pore complex (FIB-lamella data of mock infected macrophages) Method: EM (subtomogram averaging) / Resolution: 36.7 Å EMDB-52143: In situ human asymmetric subunit of nuclear pore complex (FIB-lamella data of mock infected macrophages) Method: EM (subtomogram averaging) / Resolution: 34.9 Å EMDB-52144: In situ human symmetrized composite map of nuclear pore complex (FIB-lamella data of mock infected macrophages) Method: EM (subtomogram averaging) / Resolution: 30.0 Å
Source	Homo sapiens (human)