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TitleCryoET reveals actin filaments within platelet microtubules.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 5967, Year 2024
Publish dateJul 16, 2024
AuthorsChisato Tsuji / Marston Bradshaw / Megan F Allen / Molly L Jackson / Judith Mantell / Ufuk Borucu / Alastair W Poole / Paul Verkade / Ingeborg Hers / Danielle M Paul / Mark P Dodding /
PubMed AbstractCrosstalk between the actin and microtubule cytoskeletons is important for many cellular processes. Recent studies have shown that microtubules and F-actin can assemble to form a composite structure ...Crosstalk between the actin and microtubule cytoskeletons is important for many cellular processes. Recent studies have shown that microtubules and F-actin can assemble to form a composite structure where F-actin occupies the microtubule lumen. Whether these cytoskeletal hybrids exist in physiological settings and how they are formed is unclear. Here, we show that the short-crossover Class I actin filament previously identified inside microtubules in human HAP1 cells is cofilin-bound F-actin. Lumenal F-actin can be reconstituted in vitro, but cofilin is not essential. Moreover, actin filaments with both cofilin-bound and canonical morphologies reside within human platelet microtubules under physiological conditions. We propose that stress placed upon the microtubule network during motor-driven microtubule looping and sliding may facilitate the incorporation of actin into microtubules.
External linksNat Commun / PubMed:39013865 / PubMed Central
MethodsEM (helical sym.)
Resolution20.0 Å
Structure data

EMDB-50814: Real space helical reconstruction of cofilin actin in the microtubule lumen of human platelets
Method: EM (helical sym.) / Resolution: 20.0 Å

EMDB-50845: Real space helical reconstruction of cofilin actin in the microtubule lumen of HAP1 cells
Method: EM (helical sym.) / Resolution: 20.0 Å

Source
  • Homo sapiens (human)

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