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TitleThe Asgard archaeal ESCRT-III system forms helical filaments and remodels eukaryotic-like membranes.
Journal, issue, pagesEMBO J, Vol. 44, Issue 3, Page 665-681, Year 2025
Publish dateJan 3, 2025
AuthorsNataly Melnikov / Benedikt Junglas / Gal Halbi / Dikla Nachmias / Erez Zerbib / Noam Gueta / Alexander Upcher / Ran Zalk / Carsten Sachse / Anne Bernheim-Groswasser / Natalie Elia /
PubMed AbstractThe ESCRT machinery mediates membrane remodeling in numerous processes in cells including cell division and nuclear membrane reformation. The identification of ESCRT homologs in Asgard archaea, ...The ESCRT machinery mediates membrane remodeling in numerous processes in cells including cell division and nuclear membrane reformation. The identification of ESCRT homologs in Asgard archaea, currently considered the closest prokaryotic relative of eukaryotes, implies a role for ESCRTs in the membrane remodeling processes that occurred during eukaryogenesis. Yet, the function of these distant ESCRT homologs is mostly unresolved. Here we show that Asgard ESCRT-III proteins of the Lokiarcheota self-assemble into helical filaments, a hallmark of the ESCRT system. We determined the cryo-EM structure of the filaments at 3.6 Å resolution and found that they share features of bacterial and eukaryotic ESCRT-III assemblies. Markedly, Asgard ESCRT-III filaments bound and deformed eukaryotic-like membrane vesicles. Oligonucleotides facilitated the assembly of ESCRT-III filaments and tuned the extent of membrane remodeling. The ability of Asgard archaeal ESCRTs to remodel eukaryotic-like membranes, which are fundamentally different from archaeal membranes, and the structural properties of these proteins places them at the junction between prokaryotes and eukaryotes.
External linksEMBO J / PubMed:39753954 / PubMed Central
MethodsEM (helical sym.)
Resolution3.55 Å
Structure data

50583

9fn1

EMDB-50583, PDB-9fn1:
360 A Loki CHMP4-7 rods
Method: EM (helical sym.) / Resolution: 3.55 Å

Source
  • candidatus lokiarchaeia archaeon (archaea)
KeywordsLIPID BINDING PROTEIN / ESCRT-III / membrane remodling / tubulation / archaea / Loki

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