EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure of the botulinum neurotoxin A/SV2B complex and its implications for translocation.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 1224, Year 2025
掲載日	2025年2月11日
著者	Basavraj Khanppnavar / Oneda Leka / Sushant K Pal / Volodymyr M Korkhov / Richard A Kammerer /
PubMed 要旨	Botulinum neurotoxin A1 (BoNT/A1) belongs to the most potent toxins and is used as a major therapeutic agent. Neurotoxin conformation is crucial for its translocation to the neuronal cytosol, a key ...Botulinum neurotoxin A1 (BoNT/A1) belongs to the most potent toxins and is used as a major therapeutic agent. Neurotoxin conformation is crucial for its translocation to the neuronal cytosol, a key process for intoxication that is only poorly understood. To gain molecular insights into the steps preceding toxin translocation, we determine cryo-EM structures of BoNT/A1 alone and in complex with its receptor synaptic vesicle glycoprotein 2B (SV2B). In solution, BoNT/A1 adopts a unique, semi-closed conformation. The toxin changes its structure into an open state upon receptor binding with the translocation domain (H) and the catalytic domain (LC) remote from the membrane, suggesting translocation incompatibility. Under acidic pH conditions, where translocation is initiated, receptor-bound BoNT/A1 switches back into a semi-closed conformation. This conformation brings the LC and H close to the membrane, suggesting that a translocation-competent state of the toxin is required for successful LC transport into the neuronal cytosol.
リンク	Nat Commun / PubMed:39934119 / PubMed Central
手法	EM (単粒子)
解像度	3.49 - 5.41 Å
構造データ	50135 9f1r EMDB-50135, PDB-9f1r: Cryo-EM structure of SV2B-Hc-A1 complex 手法: EM (単粒子) / 解像度: 3.67 Å 50138 9f25 EMDB-50138, PDB-9f25: Cryo-EM structure of Botulinum neurotoxin A LC-HN domain 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-50139: Cryo-EM structure of Botulinum neurotoxin A 手法: EM (単粒子) / 解像度: 3.8 Å 50146 9f2b EMDB-50146, PDB-9f2b: Focused refinement of SV2B luminal domain and BoNT/A1 complex 手法: EM (単粒子) / 解像度: 3.49 Å 50147 9f2j EMDB-50147, PDB-9f2j: Cryo-EM structure of SV2B-BoNT/A1 complex 手法: EM (単粒子) / 解像度: 3.98 Å 50151 9f3c EMDB-50151: Cryo-EM structure of SV2B-BoNT/A1 complex at pH 5.5 PDB-9f3c: Low-resolution (5.4 angstrom) cryo-EM structure of SV2B-BoNT/A1 at pH 5.5 手法: EM (単粒子) / 解像度: 5.41 Å EMDB-50154: Focused refinement of BoNT/A1 bound to SV2B at pH 5.5 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-50158: Focused refinement of SV2B-LD-HcA1 at pH 5.5 手法: EM (単粒子) / 解像度: 3.94 Å EMDB-50163: Focused refinement of BoNT/A1 LC domain bound to SV2B at pH 5.5 手法: EM (単粒子) / 解像度: 4.25 Å 50166 9f2y EMDB-50166, PDB-9f2y: Focused refinement of SV2B-LD-BoNT/A1 at pH 5.5 手法: EM (単粒子) / 解像度: 4.39 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose
由来	clostridium botulinum (ボツリヌス菌) homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN / transporter / botulinum neurotoxin / synaptic vesicle glycoprotein / TOXIN / Neurotoxin / Clostridium botulinum