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TitleCryogenic electron tomography reveals helical organization of lipoprotein lipase in storage vesicles.
Journal, issue, pagesSci Adv, Vol. 11, Issue 32, Page eadx8711, Year 2025
Publish dateAug 8, 2025
AuthorsKathryn H Gunn / Anna Wheless / Thomas Calcraft / Mark Kreutzberger / Kareem El-Houshy / Edward H Egelman / Peter B Rosenthal / Saskia B Neher /
PubMed AbstractLipoprotein lipase (LPL) is a triglyceride lipase that is contained in intracellular vesicles in an inactive storage form before secretion, but the precise structural details have not yet been ...Lipoprotein lipase (LPL) is a triglyceride lipase that is contained in intracellular vesicles in an inactive storage form before secretion, but the precise structural details have not yet been resolved. Using cryo-electron tomography (cryo-ET), we observe that LPL exists inside of storage vesicles as a filament with an 11-nanometer diameter and is packed in these vesicles in two distinct patterns. Next, we solved a 4.2-Å resolution cryo-electron microscopy (cryo-EM) structure of this 11-nanometer LPL filament using purified protein. The filament is made of repeating pairs of LPL molecules with occluded active sites, rendering the LPL inactive. The comparison of the in situ subtomogram average and the in vitro cryo-EM structure indicates that the previously uncharacterized physiological storage form of LPL is an inactive filament.
External linksSci Adv / PubMed:40768583 / PubMed Central
MethodsEM (subtomogram averaging) / EM (helical sym.)
Resolution4.2 - 32.0 Å
Structure data

EMDB-49737: Subtomogram Average of in situ Lipoprotein Lipase Filament
Method: EM (subtomogram averaging) / Resolution: 32.0 Å

49738

9nrn

EMDB-49738, PDB-9nrn:
Lipoprotein Lipase Helical Filament with 11 nm diameter
Method: EM (helical sym.) / Resolution: 4.2 Å

Source
  • Homo sapiens (human)
  • bos taurus (domestic cattle)
KeywordsHYDROLASE / Lipase / Filament / Helical Complex

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