EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanistic insights into RNA chaperoning by Ro60 and La autoantigens.
ジャーナル・号・ページ	Cell, Vol. 189, Issue 4, Page 1135-1152.e23, Year 2026
掲載日	2026年2月19日
著者	Hyeyeon Nam / Justin C Deme / Soyeong Sim / Marco Boccitto / Susan M Lea / Sandra L Wolin /
PubMed 要旨	Although ATP-independent chaperones assist RNA folding, the mechanisms by which they function remain elusive. Here, we demonstrate how two RNA chaperones collaborate to unfold misfolded noncoding ...Although ATP-independent chaperones assist RNA folding, the mechanisms by which they function remain elusive. Here, we demonstrate how two RNA chaperones collaborate to unfold misfolded noncoding RNAs (ncRNAs). The ring-shaped Ro60 protein binds the ends of misfolded ncRNAs in its cavity, whereas La stabilizes nascent ncRNAs and assists their folding. Using cryo-electron microscopy to resolve the structure of a misfolded RNA complexed with Ro60 and La, we show that La cradles the Ro60 ribonucleoprotein (RNP), with its N-terminal domain binding the RNA 3' end after it passes through the Ro60 cavity, while its C-terminal domain destabilizes structures in the misfolded RNA body. Using selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP), we show that La and Ro60 function synergistically to unfold non-native structures. As the RNAs bound by Ro60 and La include both ncRNA precursors and ncRNAs with oligouridine tails, this RNA chaperone machine may function widely to recognize misfolded and otherwise aberrant ncRNAs and assist their unfolding.
リンク	Cell / PubMed:41610850 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.5 Å
構造データ	EMDB-49327: Cryo-EM structure of Ro60/U-tailed 5S rRNA complex 手法: EM (単粒子) / 解像度: 3.5 Å 49328 9nen EMDB-49328, PDB-9nen: Cryo-EM structure of human Ro60 手法: EM (単粒子) / 解像度: 3.5 Å 49329 9nep EMDB-49329, PDB-9nep: Cryo-EM structure of Ro60/minimal misfolded pre-5S rRNA complex 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-49353: Cryo-EM structure of Ro60/La/truncated misfolded human pre-5S rRNA complex with Fab, consensus map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-49354: Cryo-EM structure of Ro60/La/truncated misfolded human pre-5S rRNA complex with Fab, focused map 手法: EM (単粒子) / 解像度: 3.3 Å 49355 9nf8 EMDB-49355, PDB-9nf8: Cryo-EM structure of Ro60/La/truncated misfolded human pre-5S rRNA complex with Fab, composite map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-49357: Cryo-EM structure of Ro60/La/minimal misfolded pre-5S rRNA complex with Fab, consensus map 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-49358: Cryo-EM structure of Ro60/La/minimal misfolded pre-5S rRNA complex with Fab, N-term La/Fab focused map 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-49359: Cryo-EM structure of Ro60/La/minimal misfolded pre-5S rRNA complex with Fab, C-term La focused map 手法: EM (単粒子) / 解像度: 3.0 Å 49360 9nfa EMDB-49360, PDB-9nfa: Cryo-EM structure of Ro60/La/minimal misfolded pre-5S rRNA complex with Fab, composite map 手法: EM (単粒子) / 解像度: 2.7 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	homo sapiens (ヒト) xenopus laevis (アフリカツメガエル)
キーワード	RNA BINDING PROTEIN/RNA / Ro60 autoantigen / RNA chaperone / RNA folding / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex / La autoantigen