EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Activation of kainate receptor GluK2-Neto2 complex.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 32, Issue 11, Page 2176-2184, Year 2025
掲載日	2025年8月22日
著者	Shanti Pal Gangwar / Maria V Yelshanskaya / Laura Y Yen / Thomas P Newton / Alexander I Sobolevsky /
PubMed 要旨	Kainate receptors (KARs) are tetrameric, ligand-gated ion channels of the ionotropic glutamate receptor family that mediate excitatory neurotransmission and modulate neuronal circuits and synaptic ...Kainate receptors (KARs) are tetrameric, ligand-gated ion channels of the ionotropic glutamate receptor family that mediate excitatory neurotransmission and modulate neuronal circuits and synaptic plasticity during development of the central nervous system. KARs are implicated in psychiatric and neurological diseases and represent a target of therapeutic intervention. Native KARs form complexes with neuropilin and tolloid-like auxiliary subunits (Neto1 and Neto2), which modulate their function, trafficking and synaptic localization. Here we present structures of rat GluK2 KAR in the apo closed state and in the open states activated by agonist kainate and positive allosteric modulator BPAM344, solved in the presence and absence of Neto2 using time-resolved cryo-electron microscopy. While the binding of Neto2 does not change the behavior of individual or dimeric ligand-binding domains (LBDs) or the ion channel, it prevents tightening of the interface between two LBD dimers during activation and slows the kinetics of deactivation. Our structures illuminate the mechanism of KAR activation and its modulation by Neto2.
リンク	Nat Struct Mol Biol / PubMed:40846810 / PubMed Central
手法	EM (単粒子)
解像度	3.22 - 4.24 Å
構造データ	48897 9n4l EMDB-48897, PDB-9n4l: Composite map for GluK2 in the apo state with asymmetric ligand binding domain 手法: EM (単粒子) / 解像度: 4.24 Å 48898 9n4m EMDB-48898, PDB-9n4m: Composite map for GluK2 in the apo state with 2-fold symmetrical ligand-binding domain 手法: EM (単粒子) / 解像度: 3.77 Å 48899 9n4n EMDB-48899, PDB-9n4n: Composite map for GluK2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate 手法: EM (単粒子) / 解像度: 3.32 Å 48900 9n4o EMDB-48900, PDB-9n4o: Composite map for GluK2-0xNeto2 in the apo state with asymmetric ligand-binding domain 手法: EM (単粒子) / 解像度: 3.6 Å 48901 9n4p EMDB-48901, PDB-9n4p: Composite map for GluK2-1xNeto2 in the apo state 手法: EM (単粒子) / 解像度: 3.58 Å 48902 9n4q EMDB-48902, PDB-9n4q: Composite map for GluK2-2xNeto2 in the apo state 手法: EM (単粒子) / 解像度: 3.48 Å 48903 9n4r EMDB-48903, PDB-9n4r: Composite map for GluK2-0xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate 手法: EM (単粒子) / 解像度: 3.48 Å 48904 9n4s EMDB-48904, PDB-9n4s: Composite map for GluK2-1xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate 手法: EM (単粒子) / 解像度: 3.34 Å 48905 9n4t EMDB-48905, PDB-9n4t: Composite map for GluK2-2xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate 手法: EM (単粒子) / 解像度: 3.22 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質YM ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-KAI: 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE / カイニン酸 ChemComp-2J9*: 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide / 3,4-ジヒドロ-4-シクロプロピル-7-フルオロ-2H-1,2,4-ベンゾチアジアジン1,1-ジオキシド
由来	rattus norvegicus (ドブネズミ)
キーワード	MEMBRANE PROTEIN / Kainate receptor / GluK2 / Ion Channel / Neto2