Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Activation of kainate receptor GluK2-Neto2 complex.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 32, Issue 11, Page 2176-2184, Year 2025
Publish date	Aug 22, 2025
Authors	Shanti Pal Gangwar / Maria V Yelshanskaya / Laura Y Yen / Thomas P Newton / Alexander I Sobolevsky /
PubMed Abstract	Kainate receptors (KARs) are tetrameric, ligand-gated ion channels of the ionotropic glutamate receptor family that mediate excitatory neurotransmission and modulate neuronal circuits and synaptic ...Kainate receptors (KARs) are tetrameric, ligand-gated ion channels of the ionotropic glutamate receptor family that mediate excitatory neurotransmission and modulate neuronal circuits and synaptic plasticity during development of the central nervous system. KARs are implicated in psychiatric and neurological diseases and represent a target of therapeutic intervention. Native KARs form complexes with neuropilin and tolloid-like auxiliary subunits (Neto1 and Neto2), which modulate their function, trafficking and synaptic localization. Here we present structures of rat GluK2 KAR in the apo closed state and in the open states activated by agonist kainate and positive allosteric modulator BPAM344, solved in the presence and absence of Neto2 using time-resolved cryo-electron microscopy. While the binding of Neto2 does not change the behavior of individual or dimeric ligand-binding domains (LBDs) or the ion channel, it prevents tightening of the interface between two LBD dimers during activation and slows the kinetics of deactivation. Our structures illuminate the mechanism of KAR activation and its modulation by Neto2.
External links	Nat Struct Mol Biol / PubMed:40846810 / PubMed Central
Methods	EM (single particle)
Resolution	3.22 - 4.24 Å
Structure data	48897 9n4l EMDB-48897, PDB-9n4l: Composite map for GluK2 in the apo state with asymmetric ligand binding domain Method: EM (single particle) / Resolution: 4.24 Å 48898 9n4m EMDB-48898, PDB-9n4m: Composite map for GluK2 in the apo state with 2-fold symmetrical ligand-binding domain Method: EM (single particle) / Resolution: 3.77 Å 48899 9n4n EMDB-48899, PDB-9n4n: Composite map for GluK2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate Method: EM (single particle) / Resolution: 3.32 Å 48900 9n4o EMDB-48900, PDB-9n4o: Composite map for GluK2-0xNeto2 in the apo state with asymmetric ligand-binding domain Method: EM (single particle) / Resolution: 3.6 Å 48901 9n4p EMDB-48901, PDB-9n4p: Composite map for GluK2-1xNeto2 in the apo state Method: EM (single particle) / Resolution: 3.58 Å 48902 9n4q EMDB-48902, PDB-9n4q: Composite map for GluK2-2xNeto2 in the apo state Method: EM (single particle) / Resolution: 3.48 Å 48903 9n4r EMDB-48903, PDB-9n4r: Composite map for GluK2-0xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate Method: EM (single particle) / Resolution: 3.48 Å 48904 9n4s EMDB-48904, PDB-9n4s: Composite map for GluK2-1xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate Method: EM (single particle) / Resolution: 3.34 Å 48905 9n4t EMDB-48905, PDB-9n4t: Composite map for GluK2-2xNeto2 in the open state, in complex with the positive allosteric modulator BPAM344 and agonist kainate Method: EM (single particle) / Resolution: 3.22 Å
Chemicals	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / phospholipidYM ChemComp-NA: Unknown entry ChemComp-KAI: 3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE / neurotransmitter, agonistYM ChemComp-2J9: 4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide
Source	rattus norvegicus (Norway rat)
Keywords	MEMBRANE PROTEIN / Kainate receptor / GluK2 / Ion Channel / Neto2