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TitleSequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
Journal, issue, pagesCell Host Microbe, Vol. 33, Issue 4, Page 484-497.e6, Year 2025
Publish dateApr 9, 2025
AuthorsEmily G Armbruster / Phoolwanti Rani / Jina Lee / Niklas Klusch / Joshua Hutchings / Lizbeth Y Hoffman / Hannah Buschkaemper / Eray Enustun / Benjamin A Adler / Koe Inlow / Arica R VanderWal / Madelynn Y Hoffman / Daksh Daksh / Ann Aindow / Amar Deep / Zaida K Rodriguez / Chase J Morgan / Majid Ghassemian / Thomas G Laughlin / Emeric Charles / Brady F Cress / David F Savage / Jennifer A Doudna / Kit Pogliano / Kevin D Corbett / Elizabeth Villa / Joe Pogliano /
PubMed AbstractMany eukaryotic viruses require membrane-bound compartments for replication, but no such organelles are known to be formed by prokaryotic viruses. Bacteriophages of the Chimalliviridae family ...Many eukaryotic viruses require membrane-bound compartments for replication, but no such organelles are known to be formed by prokaryotic viruses. Bacteriophages of the Chimalliviridae family sequester their genomes within a phage-generated organelle, the phage nucleus, which is enclosed by a lattice of the viral protein ChmA. We show that inhibiting phage nucleus formation arrests infections at an early stage in which the injected phage genome is enclosed within a membrane-bound early phage infection (EPI) vesicle. Early phage genes are expressed from the EPI vesicle, demonstrating its functionality as a prokaryotic, transcriptionally active, membrane-bound organelle. We also show that the phage nucleus is essential, with genome replication beginning after the injected DNA is transferred from the EPI vesicle to the phage nucleus. Our results show that Chimalliviridae require two sophisticated subcellular compartments of distinct compositions and functions that facilitate successive stages of the viral life cycle.
External linksCell Host Microbe / PubMed:40168997
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution10.0 - 33.68 Å
Structure data

EMDB-48856: 70S Ribosome of Goslar infected WT E. coli
Method: EM (subtomogram averaging) / Resolution: 33.68 Å

EMDB-48875: 70S Ribosome of Goslar infected chmA KD E. coli
Method: EM (subtomogram averaging) / Resolution: 20.56 Å

EMDB-48876: 70S Ribosome of Goslar infected chmA KD E. coli
Method: EM (subtomogram averaging) / Resolution: 21.33 Å

EMDB-49120: In situ cryoET of an EPI vesicle in a Goslar infected chmA KD E. coli cell 90 mpi
Method: EM (tomography) / Resolution: 18.0 Å

EMDB-49121: In situ cryoET of an EPI vesicle in a Goslar infected chmA KD E. coli cell 90 mpi
Method: EM (tomography) / Resolution: 15.0 Å

EMDB-49122: In situ cryoET of an EPI vesicle in a Goslar infected chmA KD E. coli cell 30 mpi
Method: EM (tomography) / Resolution: 10.0 Å

EMDB-49123: In situ cryoET of an EPI vesicle in a Goslar infected WT E. coli cell 1 mpi
Method: EM (tomography) / Resolution: 10.0 Å

Source
  • Escherichia coli (E. coli)

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