EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The twist-and-squeeze activation of CARF-fused adenosine deaminase by cyclic oligoadenylates.
ジャーナル・号・ページ	EMBO J, Vol. 44, Issue 23, Page 6919-6943, Year 2025
掲載日	2025年10月17日
著者	Charlisa Whyms / Yu Zhao / Doreen Addo-Yobo / Huan He / Arthur Carl Whittington / Despoina Trasanidou / Carl Raymund P Salazar / Raymond H J Staals / Hong Li /
PubMed 要旨	The recently identified CARF (CRISPR-associated Rossman-fold) family of proteins play a critical role in prokaryotic defense, mediating cOA (cyclic oligoadenylate)-stimulated ancillary immune ...The recently identified CARF (CRISPR-associated Rossman-fold) family of proteins play a critical role in prokaryotic defense, mediating cOA (cyclic oligoadenylate)-stimulated ancillary immune responses in the type III CRISPR-Cas systems. Whereas most previously characterized CARF proteins contain nucleic acids or protein degradation effectors, a subset of the family, including the CARF-fused adenosine deaminase (ADA) (Cad1), has recently been shown to convert ATP to ITP. The enzymatic mechanism and the activation process of Cad1, however, remain incompletely understood. Here we present biochemical and structural analyses of a ring nuclease Cad1, revealing its substrate binding specificity and a sequential activation process by cOAs. Despite an overall structural similarity to canonical ADA enzymes, the ADA domain of Cad1 possesses unique structural features that confer a specificity for ATP. Supported by mutational analysis, our structural work demonstrates an allosteric link between the cOA-binding CARF and the ADA domain through a protein network within the hexameric enzyme assembly. Binding of a cA4 molecule to paired CARF domains induces a twisting of the linked ADA domains around one another, which remodels their active sites and alters interactions with neighboring ADA domains, thereby driving a sequential conformational activation mechanism.
リンク	EMBO J / PubMed:41107544 / PubMed Central
手法	EM (単粒子)
解像度	2.31 - 3.17 Å
構造データ	EMDB-47886: CRISPR-associated deaminase Cad1 in cA4 bound form, symmetry expanded dimer, consensus map 手法: EM (単粒子) / 解像度: 2.36 Å EMDB-47887: CRISPR-associated deaminase Cad1 in cA4 bound form, symmetry expanded dimer, CARF domain focus refined map 手法: EM (単粒子) / 解像度: 2.53 Å EMDB-47888: CRISPR-associated deaminase Cad1 in cA4 bound form, symmetry expanded dimer, deaminase domain focus refined map 手法: EM (単粒子) / 解像度: 2.31 Å 47890 9ebt EMDB-47890, PDB-9ebt: CRISPR-associated deaminase Cad1 in cA4 bound form, symmetry expanded dimer, refined against a composite map 手法: EM (単粒子) / 解像度: 2.36 Å 48116 9eka EMDB-48116, PDB-9eka: CRISPR-associated deaminase Cad1 in Apo form 手法: EM (単粒子) / 解像度: 3.1 Å 48405 9mmw EMDB-48405, PDB-9mmw: CRISPR-associated deaminase Cad1 in cA4 bound in hexamer form refined against the consensus map 手法: EM (単粒子) / 解像度: 2.36 Å 70417 9of1 EMDB-70417: CryoEM structure of Cad1 in App form, symmetry expanded dimer, refined against a composite map PDB-9of1: CryoEM structure of Cad1 in Apo form, symmetry expanded dimer, refined against a composite map 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-70419: Consensus map of Cad1 in App form 手法: EM (単粒子) / 解像度: 2.7 Å 70422 9ofb EMDB-70422, PDB-9ofb: CryoEM structure of Cad1 bound with cA4 and ATP, symmetry expanded dimer refined against a composite map 手法: EM (単粒子) / 解像度: 2.68 Å EMDB-70423: Focused map on CARF domain of Cad1 in Apo form 手法: EM (単粒子) / 解像度: 2.87 Å EMDB-70424: Focused map for the ADA domain of Cad1 in Apo form 手法: EM (単粒子) / 解像度: 2.55 Å EMDB-70425: Consensus map of Cad1 bound with cA4 and ATP 手法: EM (単粒子) / 解像度: 2.68 Å EMDB-70426: Focused map of the CARF domain of Cad1 bound with cA4 and ATP 手法: EM (単粒子) / 解像度: 2.54 Å EMDB-70427: Focused map of the ADA domain of Cad1 bound with cA4 and ATP 手法: EM (単粒子) / 解像度: 2.45 Å 70428 9ofc EMDB-70428, PDB-9ofc: CryoEM structure of Cad1 bound with cA4 and ATP, hexamer with three intact dimers 手法: EM (単粒子) / 解像度: 3.17 Å 70429 9ofd EMDB-70429, PDB-9ofd: CryoEM structure of Cad1 bound with cA4 and ATP, hexamer with one intact dimer 手法: EM (単粒子) / 解像度: 2.86 Å 70430 9ofe EMDB-70430, PDB-9ofe: CryoEM structure of Cad1 bound with cA4 and ATP, hexamer with two intact dimers 手法: EM (単粒子) / 解像度: 2.68 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH*: WATER
由来	thermoanaerobaculum aquaticum (バクテリア)
キーワード	SIGNALING PROTEIN / CRISPR-associated CARF ring nuclease / Cad1 / CRISPR-associated adenosine deaminase / S-adenosylmethionine / HYDROLASE / ANTIVIRAL PROTEIN/RNA / ANTIVIRAL PROTEIN / ANTIVIRAL PROTEIN-RNA complex / IMMUNE SYSTEM / CRISPR-associated Rossman-fold (CARF) / ATP deaminase / CRISPR immunity / cooperative activation