EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures and functions of the limited natural polyclonal antibody response to parvovirus infection.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 122, Issue 8, Page e2423460122, Year 2025
掲載日	2025年2月25日
著者	Oluwafemi F Adu / Hyunwook Lee / Simon P Früh / Marta V Schoenle / Wendy S Weichert / Andrew I Flyak / Susan L Hafenstein / Colin R Parrish /
PubMed 要旨	Host antibody responses are key components in the protection of animals against pathogens, yet the defining properties of viral antigens and induction of B cell responses that result in varied ...Host antibody responses are key components in the protection of animals against pathogens, yet the defining properties of viral antigens and induction of B cell responses that result in varied protection are still poorly understood. Parvoviruses are simple molecular structures that display 60 repeated motifs on their capsid surface, and rapidly induce strong antibody responses that protect animals from infection. We recently showed that following canine parvovirus infection of its natural host, the polyclonal response in the sera contained only two or three dominant antibodies that bound two epitopes on the capsid. Here, we characterize key antibodies present in that immune response, identifying their sequences, defining their binding properties on the capsid by cryoelectron microscopic (cryoEM) analysis, and testing their effects on viral infectivity. Two antibodies sharing the same heavy chain bound to the side of the capsid threefold spike (B-site), while another distinct antibody bound close to the threefold axis (A-site). The epitopes of these antibodies overlapped the binding site of the host receptor, the transferrin receptor type-1, but to varying degrees. The antibodies varied widely in their neutralization efficiencies as either immunoglobulins (IgGs) or monomeric antigen-binding fragments (Fabs), which was consistent with their ability to compete for the receptor. The monoclonal antibodies characterized here matched the structures from the cryoEM analysis of polyclonal sera, including those present in a different dog than the monoclonal source. This shows that after infection, a focused response to the viral antigen is produced that protects against infection.
リンク	Proc Natl Acad Sci U S A / PubMed:39951487 / PubMed Central
手法	EM (単粒子)
解像度	1.81 - 4.1 Å
構造データ	47538 9e60 EMDB-47538, PDB-9e60: Canine parvovirus subtype 2a empty capsid in complex with Fab fragments of Mab 7C8 手法: EM (単粒子) / 解像度: 2.65 Å 47693 9e7w EMDB-47693, PDB-9e7w: Canine parvovirus subtype 2a empty capsid in complex with Fab fragments of Mab 3G6 手法: EM (単粒子) / 解像度: 3.7 Å 47694 9e7x EMDB-47694, PDB-9e7x: Canine parvovirus subtype 2a empty capsid in complex with Fab fragments of Mab 2C5 手法: EM (単粒子) / 解像度: 3.2 Å 47696 9e7z EMDB-47696, PDB-9e7z: Canine parvovirus subtype 2a empty capsid in complex with Fab fragments of Mab 3G6 手法: EM (単粒子) / 解像度: 2.02 Å 47697 9e80 EMDB-47697, PDB-9e80: Canine parvovirus subtype 2a empty capsid in complex with Fab fragments of Mab 2C5 手法: EM (単粒子) / 解像度: 1.81 Å EMDB-47703: Sub-volume reconstruction of cFab 3G6 手法: EM (単粒子) / 解像度: 2.91 Å EMDB-47704: Sub-volume map of cFab 2C5 手法: EM (単粒子) / 解像度: 2.8 Å 47711 9e89 EMDB-47711, PDB-9e89: CPV2a capsid complexed with scFv2 手法: EM (単粒子) / 解像度: 3.5 Å 47717 9e8d EMDB-47717, PDB-9e8d: CPV2a capsid complexed with scFv1 手法: EM (単粒子) / 解像度: 4.1 Å
由来	canine parvovirus 2a (ウイルス) canis lupus familiaris (イヌ) canine parvovirus 2b (ウイルス)
キーワード	VIRUS / Antibody / Complex / VIRUS/IMMUNE SYSTEM / VIRUS-IMMUNE SYSTEM complex