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TitleStructural insights into brain thyroid hormone transport via MCT8 and OATP1C1.
Journal, issue, pagesCell, Vol. 188, Issue 20, Page 5576-5588.e17, Year 2025
Publish dateJul 15, 2025
AuthorsYunhui Ge / Tongyi Dou / Thu Uyen Nguyen / Gaya P Yadav / Theodore G Wensel / Jiansen Jiang / Pengxiang Huang /
PubMed AbstractAdequate delivery of thyroid hormones to the brain is crucial for normal neurological development. MCT8 and OATP1C1, two solute carrier (SLC) transporters, mediate the passage of thyroid hormones ...Adequate delivery of thyroid hormones to the brain is crucial for normal neurological development. MCT8 and OATP1C1, two solute carrier (SLC) transporters, mediate the passage of thyroid hormones across the blood-brain barrier and into the central nervous system. Mutations in MCT8 result in Allan-Herndon-Dudley syndrome (AHDS), an X-linked birth defect characterized by neurodevelopmental impairments and peripheral hyperthyroidism, whereas OATP1C1 deficiency is linked to brain hypometabolism and progressive neurodegeneration. Here, we report cryoelectron microscopy (cryo-EM) structures of MCT8 and OATP1C1 bound with the active thyroid hormone triiodothyronine (T3) and the prohormone thyroxine (T4) at 2.9 and 2.3 Å resolutions, respectively. Combined with functional studies, we elucidate their distinct thyroid hormone recognition and transport mechanisms and explain disease mutations. Although extracellular allosteric sites are not a common feature of SLC transporters, we identify one in OATP1C1. Collectively, these findings illuminate key aspects of thyroid hormone transport, a fundamental process in development and disease.
External linksCell / PubMed:40680733 / PubMed Central
MethodsEM (single particle)
Resolution2.3 - 3.13 Å
Structure data

47274

9dwy

EMDB-47274, PDB-9dwy:
Cryo-EM structure of human MCT8 in complex with thyroid hormone T3
Method: EM (single particle) / Resolution: 2.9 Å

47293

9dxo

EMDB-47293, PDB-9dxo:
Cryo-EM structure of human OATP1C1 F240A mutant in complex with estrone 3-sulfate
Method: EM (single particle) / Resolution: 2.6 Å

47294

9dxp

EMDB-47294, PDB-9dxp:
Cryo-EM structure of human OATP1C1 in complex with thyroid hormone T4
Method: EM (single particle) / Resolution: 2.3 Å

48553

9mr5

EMDB-48553, PDB-9mr5:
Cryo-EM structure of human OATP1C1 F240A mutant in complex with estrone 3-glucuronide
Method: EM (single particle) / Resolution: 3.13 Å

Chemicals

ChemComp-T3:
3,5,3'TRIIODOTHYRONINE / hormone*YM

ChemComp-FY5:
estrone 3-sulfate / medication, hormone*YM

ChemComp-T44:
3,5,3',5'-TETRAIODO-L-THYRONINE / hormone*YM

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-HOH:
WATER

ChemComp-E3G:
ESTRONE BETA-D-GLUCURONIDE

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / SLC transporter / thyroid hormone / E1S / E1G

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