EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transport and InsP gating mechanisms of the human inorganic phosphate exporter XPR1.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 2770, Year 2025
掲載日	2025年3月20日
著者	Qinyu Zhu / Madeleine F Yaggi / Nikolaus Jork / Henning J Jessen / Melinda M Diver /
PubMed 要旨	Inorganic phosphate (Pi) has essential metabolic and structural roles in living organisms. The Pi exporter, XPR1/SLC53A1, is critical for cellular Pi homeostasis. When intercellular Pi is high, cells ...Inorganic phosphate (Pi) has essential metabolic and structural roles in living organisms. The Pi exporter, XPR1/SLC53A1, is critical for cellular Pi homeostasis. When intercellular Pi is high, cells accumulate inositol pyrophosphate (1,5-InsP), a signaling molecule required for XPR1 function. Inactivating XPR1 mutations lead to brain calcifications, causing neurological symptoms including movement disorders, psychosis, and dementia. Here, cryo-electron microscopy structures of dimeric XPR1 and functional characterization delineate the substrate translocation pathway and how InsP initiates Pi transport. Binding of InsP to XPR1, but not the related inositol polyphosphate InsP, rigidifies the intracellular SPX domains, with InsP bridging the dimers and SPX and transmembrane domains. Locked in this state, the C-terminal tail is sequestered, revealing the entrance to the transport pathway, thus explaining the obligate roles of the SPX domain and InsP. Together, these findings advance our understanding of XPR1 transport activity and expand opportunities for rationalizing disease mechanisms and therapeutic intervention.
リンク	Nat Commun / PubMed:40113814 / PubMed Central
手法	EM (単粒子)
解像度	2.52 - 3.3 Å
構造データ	EMDB-47207: Structure of the phosphate exporter XPR1/SLC53A1, high Pi and InsP6-bound 手法: EM (単粒子) / 解像度: 3.3 Å 47208 9dvj EMDB-47208: Structure of the phosphate exporter XPR1/SLC53A1, apo state PDB-9dvj: "Structure of the phosphate exporter XPR1/SLC53A1 手法: EM (単粒子) / 解像度: 2.52 Å 47209 9dvk EMDB-47209: Structure of the phosphate exporter XPR1/SLC53A1, apo state, rotated dimer PDB-9dvk: Structure of the phosphate exporter XPR1/SLC53A1, rotated dimer 手法: EM (単粒子) / 解像度: 3.06 Å 47210 9dvl EMDB-47210, PDB-9dvl: Structure of the phosphate exporter XPR1/SLC53A1, InsP6-supplemented 手法: EM (単粒子) / 解像度: 2.97 Å 47211 9dvm EMDB-47211: Structure of the phosphate exporter XPR1/SLC53A1, InsP8-bound, inward-open/occluded state PDB-9dvm: Structure of the phosphate exporter XPR1/SLC53A1, InsP8-bound, intracellular gate open/intracellular gate closed state 手法: EM (単粒子) / 解像度: 2.92 Å 47212 9dvn EMDB-47212: Structure of the phosphate exporter XPR1/SLC53A1, InsP8-bound, occluded state PDB-9dvn: Structure of the phosphate exporter XPR1/SLC53A1, InsP8-bound, intracellular gate closed state 手法: EM (単粒子) / 解像度: 2.75 Å 47213 9dvo EMDB-47213: Structure of the phosphate exporter XPR1/SLC53A1, Pi and InsP8-bound, inward-open/occluded state PDB-9dvo: Structure of the phosphate exporter XPR1/SLC53A1, Pi and InsP8-bound, intracellular gate open/intracellular gate closed state 手法: EM (単粒子) / 解像度: 3.1 Å 47214 9dvp EMDB-47214: Structure of the phosphate exporter XPR1/SLC53A1, Pi and InsP8-bound, occluded state PDB-9dvp: Structure of the phosphate exporter XPR1/SLC53A1, Pi and InsP8-bound, intracellular gate closed state 手法: EM (単粒子) / 解像度: 2.81 Å
化合物	ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-CPL: 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-I8P: (1R,3S,4R,5S,6R)-2,4,5,6-tetrakis(phosphonooxy)cyclohexane-1,3-diyl bis[trihydrogen (diphosphate)] / D-myo-イノシト-ル2,3,4,6-テトラキスりん酸1,5-ビス二りん酸 ChemComp-HOH*: WATER
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN / Inorganic phosphate exporter