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TitleEncapsulation mechanisms and structural studies of GRM2 bacterial microcompartment particles.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 388, Year 2020
Publish dateJan 20, 2020
AuthorsGints Kalnins / Eva-Emilija Cesle / Juris Jansons / Janis Liepins / Anatolij Filimonenko / Kaspars Tars /
PubMed AbstractBacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, ...Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated in BMCs, an improved understanding of BMC shell assembly and encapsulation processes could be useful for synthetic biology applications. Here we report the isolation and recombinant expression of BMC structural genes from the Klebsiella pneumoniae GRM2 locus, the investigation of mechanisms behind encapsulation of the core enzymes, and the characterization of shell particles by cryo-EM. We conclude that the enzymatic core is encapsulated in a hierarchical manner and that the CutC choline lyase may play a secondary role as an adaptor protein. We also present a cryo-EM structure of a pT = 4 quasi-symmetric icosahedral shell particle at 3.3 Å resolution, and demonstrate variability among the minor shell forms.
External linksNat Commun / PubMed:31959751 / PubMed Central
MethodsEM (single particle)
Resolution3.28 - 9.64 Å
Structure data

4595

6qn1

EMDB-4595, PDB-6qn1:
T=4 quasi-symmetric bacterial microcompartment particle
Method: EM (single particle) / Resolution: 3.28 Å

EMDB-4596:
T=4, Q=6 quasi-symmetric bacterial microcompartment particle
Method: EM (single particle) / Resolution: 9.64 Å

EMDB-4597:
T=4 quasi symmetric bacterial microcompartment particle with one missing pentameric EutN unit
Method: EM (single particle) / Resolution: 8.75 Å

Source
  • klebsiella pneumoniae (bacteria)
KeywordsSTRUCTURAL PROTEIN / bacterial microcompartment / protein shell / GRM2 type microcompartment / EutN / ccmK / PduA

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