EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Autoinhibition imposed by a large conformational switch of INO80 regulates nucleosome positioning.
ジャーナル・号・ページ	Science, Vol. 389, Issue 6757, Page eadr3831, Year 2025
掲載日	2025年7月17日
著者	Upneet Kaur / Hao Wu / Yifan Cheng / Geeta J Narlikar /
PubMed 要旨	Increasing the flanking DNA from 40 to 80 base pairs (bp) causes ~100-fold faster nucleosome sliding by INO80. A prevalent hypothesis posits that the Arp8 module within INO80 enables a ruler-like ...Increasing the flanking DNA from 40 to 80 base pairs (bp) causes ~100-fold faster nucleosome sliding by INO80. A prevalent hypothesis posits that the Arp8 module within INO80 enables a ruler-like activity. Using cryogenic electron microscopy, we show that on nucleosomes with 40 bp of flanking DNA, the Arp8 module rotates 180° away from the DNA. Deleting the Arp8 module enables rapid sliding irrespective of flanking DNA length. Thus, rather than enabling a ruler-like activity, the Arp8 module acts as a brake on INO80 remodeling when flanking DNA is short. This autoinhibition-based mechanism has broad implications for understanding how primitive nucleosome mobilization enzymes may have evolved into sophisticated remodelers.
リンク	Science / PubMed:40674492 / PubMed Central
手法	EM (単粒子)
解像度	2.55 - 4.18 Å
構造データ	45361 9c9g EMDB-45361, PDB-9c9g: S.c INO80 in complex with S.c 0/80 nucleosome 手法: EM (単粒子) / 解像度: 2.91 Å 45369 9c9s EMDB-45369, PDB-9c9s: S.c INO80 in complex with S.c 0/40 nucleosome, Class 1 手法: EM (単粒子) / 解像度: 3.09 Å 45370 9c9t EMDB-45370, PDB-9c9t: S.c INO80 in complex with S.c 0/40 nucleosome, Class 2 手法: EM (単粒子) / 解像度: 3.16 Å 45375 9c9x EMDB-45375, PDB-9c9x: S.c INO80 in complex with Xenopus 0/80 nucleosome, Nucleosome 手法: EM (単粒子) / 解像度: 2.83 Å 45377 9c9z EMDB-45377, PDB-9c9z: S.c INO80 in complex with Xenopus 0/80 nucleosome, INO80 Core 手法: EM (単粒子) / 解像度: 2.55 Å 45397 9can EMDB-45397, PDB-9can: S.c INO80 in complex with Xenopus 0/40 nucleosome 手法: EM (単粒子) / 解像度: 3.3 Å 45403 9cat EMDB-45403, PDB-9cat: DeltaArp8 INO80 bound to S.c 0/40 nucleosome, INO80 Core Module 手法: EM (単粒子) / 解像度: 2.9 Å 45404 9cau EMDB-45404, PDB-9cau: DeltaArp8 INO80 bound to S.c 0/40 nucleosome, Nucleosome 手法: EM (単粒子) / 解像度: 4.18 Å 45418 9cb7 EMDB-45418, PDB-9cb7: DeltaNhp10 INO80 bound to S.c 0/40 nucleosome, Ino80-Nucleosome 手法: EM (単粒子) / 解像度: 4.04 Å 45441 9ccd EMDB-45441, PDB-9ccd: DeltaNhp10 INO80 bound to S.c 0/40 nucleosome, INO80 core module 手法: EM (単粒子) / 解像度: 3.01 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM
由来	saccharomyces cerevisiae (パン酵母) xenopus laevis (アフリカツメガエル) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN/DNA / Chromatin Remodeler / nucleosome / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex