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TitleRole of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins.
Journal, issue, pagesMol Cell, Vol. 84, Issue 17, Page 3302-3319.e11, Year 2024
Publish dateSep 5, 2024
AuthorsKatharine R Page / Vy N Nguyen / Tino Pleiner / Giovani Pinton Tomaleri / Maxine L Wang / Alina Guna / Masami Hazu / Ting-Yu Wang / Tsui-Fen Chou / Rebecca M Voorhees /
PubMed AbstractMammalian membrane proteins perform essential physiologic functions that rely on their accurate insertion and folding at the endoplasmic reticulum (ER). Using forward and arrayed genetic screens, we ...Mammalian membrane proteins perform essential physiologic functions that rely on their accurate insertion and folding at the endoplasmic reticulum (ER). Using forward and arrayed genetic screens, we systematically studied the biogenesis of a panel of membrane proteins, including several G-protein-coupled receptors (GPCRs). We observed a central role for the insertase, the ER membrane protein complex (EMC), and developed a dual-guide approach to identify genetic modifiers of the EMC. We found that the back of Sec61 (BOS) complex, a component of the multipass translocon, was a physical and genetic interactor of the EMC. Functional and structural analysis of the EMC⋅BOS holocomplex showed that characteristics of a GPCR's soluble domain determine its biogenesis pathway. In contrast to prevailing models, no single insertase handles all substrates. We instead propose a unifying model for coordination between the EMC, the multipass translocon, and Sec61 for the biogenesis of diverse membrane proteins in human cells.
External linksMol Cell / PubMed:39173640
MethodsEM (single particle)
Resolution3.65 - 8.85 Å
Structure data

EMDB-45293: Structure of the human BOS complex in GDN
Method: EM (single particle) / Resolution: 8.85 Å

45294

9c7u

EMDB-45294, PDB-9c7u:
Structure of the human truncated BOS complex in GDN
Method: EM (single particle) / Resolution: 3.65 Å

45295

9c7v

EMDB-45295, PDB-9c7v:
Structure of the human BOS:human EMC complex in GDN
Method: EM (single particle) / Resolution: 6.6 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / membrane protein biogenesis / membrane protein complex

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