EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.
ジャーナル・号・ページ	Science, Vol. 363, Issue 6428, Page 710-714, Year 2019
掲載日	2019年2月15日
著者	Sebastian M Fica / Chris Oubridge / Max E Wilkinson / Andrew J Newman / Kiyoshi Nagai /
PubMed 要旨	During exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a ...During exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a conformation stabilized by Prp18 and Prp8. Here we present the 3.3-angstrom cryo-electron microscopy structure of a human postcatalytic spliceosome just after exon ligation. The 3'SS docks at the active site through conserved RNA interactions in the absence of Prp18. Unexpectedly, the metazoan-specific FAM32A directly bridges the 5'-exon and intron 3'SS of pre-mRNA and promotes exon ligation, as shown by functional assays. CACTIN, SDE2, and NKAP-factors implicated in alternative splicing-further stabilize the catalytic conformation of the spliceosome during exon ligation. Together these four proteins act as exon ligation factors. Our study reveals how the human spliceosome has co-opted additional proteins to modulate a conserved RNA-based mechanism for 3'SS selection and to potentially fine-tune alternative splicing at the exon ligation stage.
リンク	Science / PubMed:30705154 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 5.9 Å
構造データ	4525 6qdv EMDB-4525: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map PDB-6qdv: Human post-catalytic P complex spliceosome 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-4526: Human post-catalytic spliceosome (P complex) stalled with DHX8 S717A mutant, overall map 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-4527: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, focused refinement on core 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-4528: Human post-catalytic spliceosome (P complex) stalled with DHX8 S717A mutant, focused refinement on core 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-4529: Human post-catalytic spliceosome (P complex), focused refinement on Aquarius and SYF1 手法: EM (単粒子) / 解像度: 5.9 Å EMDB-4530: Human post-catalytic spliceosome (P complex), focused refinement on Brr2 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-4532: Human post-catalytic spliceosome (P complex), focused refinement on DHX8 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-4533: Human post-catalytic spliceosome (P complex), focused refinement on Prp19 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-4534: Human post-catalytic spliceosome (P complex), focused refinement on U2 snRNP 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-4535: Human post-catalytic spliceosome (P complex), focused refinement on U5 Sm 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-ZN: Unknown entry ChemComp-IHP: INOSITOL HEXAKISPHOSPHATE / myo-イノシト-ル六りん酸
由来	homo sapiens (ヒト) human adenovirus 2 (ヒトアデノウイルス)
キーワード	SPLICING / spliceosome / RNA / complex