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| Title | The molecular architecture of the nuclear basket. |
|---|---|
| Journal, issue, pages | Cell, Vol. 187, Issue 19, Page 5267-5281.e13, Year 2024 |
| Publish date | Sep 19, 2024 |
 Authors | Digvijay Singh / Neelesh Soni / Joshua Hutchings / Ignacia Echeverria / Farhaz Shaikh / Madeleine Duquette / Sergey Suslov / Zhixun Li / Trevor van Eeuwen / Kelly Molloy / Yi Shi / Junjie Wang / Qiang Guo / Brian T Chait / Javier Fernandez-Martinez / Michael P Rout / Andrej Sali / Elizabeth Villa /    |
| PubMed Abstract | The nuclear pore complex (NPC) is the sole mediator of nucleocytoplasmic transport. Despite great advances in understanding its conserved core architecture, the peripheral regions can exhibit ...The nuclear pore complex (NPC) is the sole mediator of nucleocytoplasmic transport. Despite great advances in understanding its conserved core architecture, the peripheral regions can exhibit considerable variation within and between species. One such structure is the cage-like nuclear basket. Despite its crucial roles in mRNA surveillance and chromatin organization, an architectural understanding has remained elusive. Using in-cell cryo-electron tomography and subtomogram analysis, we explored the NPC's structural variations and the nuclear basket across fungi (yeast; S. cerevisiae), mammals (mouse; M. musculus), and protozoa (T. gondii). Using integrative structural modeling, we computed a model of the basket in yeast and mammals that revealed how a hub of nucleoporins (Nups) in the nuclear ring binds to basket-forming Mlp/Tpr proteins: the coiled-coil domains of Mlp/Tpr form the struts of the basket, while their unstructured termini constitute the basket distal densities, which potentially serve as a docking site for mRNA preprocessing before nucleocytoplasmic transport. |
 External links | Cell / PubMed:39127037 / PubMed Central |
| Methods | EM (subtomogram averaging) |
| Resolution | 29.0 - 64.0 Å |
| Structure data |  EMDB-44372: In-cell Saccharomyces cerevisiae nuclear pore complex with single nuclear ring  EMDB-44377: In-cell Saccharomyces cerevisiae nuclear pore complex with double nuclear ring and basket  EMDB-44379: In-cell Mus musculus nuclear pore complex with nuclear basket  EMDB-44381: In-cell Toxoplasma gondii nuclear pore complex  EMDB-45197: In-cell Saccharomyces cerevisiae symmetry-expanded nuclear pore complex with double nuclear ring and basket  EMDB-45198: In-cell Saccharomyces cerevisiae symmetry-expanded nuclear pore complex with single nuclear ring  EMDB-45199: In-cell Saccharomyces cerevisiae nuclear pore complex cytoplasmic ring focused refinement  EMDB-45200: In-cell Saccharomyces cerevisiae nuclear pore complex inner ring focused refinement  EMDB-45201: In-cell Saccharomyces cerevisiae nuclear pore complex single nuclear ring focused refinement  EMDB-45202: In-cell Saccharomyces cerevisiae nuclear pore complex double nuclear ring focused refinement  EMDB-45203: In-cell Saccharomyces cerevisiae nuclear pore complex nuclear basket focused refinement  EMDB-45204: In-cell Saccharomyces cerevisiae nuclear pore complex membrane focused refinement for single nuclear ring  EMDB-45205: In-cell Saccharomyces cerevisiae nuclear pore complex membrane focused refinement for double nuclear ring  EMDB-45216: In-cell Mus musculus nuclear pore complex with nuclear basket consensus map  EMDB-45219: In-cell Mus musculus nuclear pore complex cytoplasmic ring focused refinement  EMDB-45220: In-cell Mus musculus nuclear pore complex inner ring focused refinement  EMDB-45222: In-cell Mus musculus nuclear pore complex nuclear ring focused refinement  EMDB-45223: In-cell Mus musculus nuclear pore complex basket focused refinement  EMDB-45227: In-cell Mus musculus nuclear pore complex membrane focused refinement  EMDB-45228: In-cell Toxoplasma gondii symmetry-expanded nuclear pore complex  EMDB-45229: In-cell Toxoplasma gondii nuclear pore complex cytoplasmic ring focused refinement  EMDB-45230: In-cell Toxoplasma gondii nuclear pore complex inner ring focused refinement  EMDB-45231: In-cell Toxoplasma gondii nuclear pore complex nuclear ring focused refinement  EMDB-45232: In-cell Toxoplasma gondii nuclear pore complex membrane focused refinement  EMDB-45233: In-cell Toxoplasma gondii nuclear pore complex lumenal ring focused refinement  EMDB-45255: In-cell Saccharomyces cerevisiae C8-symmetrised nuclear pore complex consensus map  EMDB-45256: In-cell Saccharomyces cerevisiae symmetry-expanded nuclear pore complex consensus map  EMDB-45257: In-cell Mus musculus nuclear pore complex with nuclear basket consensus map  EMDB-45258: In-cell Mus musculus symmetry-expanded nuclear pore complex with nuclear basket consensus map  EMDB-45259: In-cell Toxoplasma gondii C8-symmetrised nuclear pore complex consensus map  EMDB-45260: In-cell Toxoplasma gondii symmetry-expanded nuclear pore complex consensus map |
| Source |
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Saccharomyces cerevisiae (brewer's yeast)
Mus musculus (house mouse)
Toxoplasma gondii RH (eukaryote)