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TitleThe cryo-electron microscopy supramolecular structure of the bacterial stressosome unveils its mechanism of activation.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 3005, Year 2019
Publish dateJul 8, 2019
AuthorsAllison H Williams / Adam Redzej / Nathalie Rolhion / Tiago R D Costa / Aline Rifflet / Gabriel Waksman / Pascale Cossart /
PubMed AbstractHow the stressosome, the epicenter of the stress response in bacteria, transmits stress signals from the environment has remained elusive. The stressosome consists of multiple copies of three ...How the stressosome, the epicenter of the stress response in bacteria, transmits stress signals from the environment has remained elusive. The stressosome consists of multiple copies of three proteins RsbR, RsbS and RsbT, a kinase that is important for its activation. Using cryo-electron microscopy, we determined the atomic organization of the Listeria monocytogenes stressosome at 3.38 Å resolution. RsbR and RsbS are organized in a 60-protomers truncated icosahedron. A key phosphorylation site on RsbR (T209) is partially hidden by an RsbR flexible loop, whose "open" or "closed" position could modulate stressosome activity. Interaction between three glutamic acids in the N-terminal domain of RsbR and the membrane-bound mini-protein Prli42 is essential for Listeria survival to stress. Together, our data provide the atomic model of the stressosome core and highlight a loop important for stressosome activation, paving the way towards elucidating the mechanism of signal transduction by the stressosome in bacteria.
External linksNat Commun / PubMed:31285450 / PubMed Central
MethodsEM (single particle)
Resolution3.37 - 4.21 Å
Structure data

4508

6qcm

EMDB-4508, PDB-6qcm:
Cryo em structure of the Listeria stressosome
Method: EM (single particle) / Resolution: 4.21 Å

EMDB-4510:
Cryo em structure of the Listeria stressosome
Method: EM (single particle) / Resolution: 3.37 Å

Source
  • listeria monocytogenes egd-e (bacteria)
  • Listeria monocytogenes (bacteria)
KeywordsANTIMICROBIAL PROTEIN / Stressosome complex / stress response machine / Bacteria stress sensor

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