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TitleA higher order PUF complex is central to regulation of C. elegans germline stem cells.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 123, Year 2025
Publish dateJan 2, 2025
AuthorsChen Qiu / Sarah L Crittenden / Brian H Carrick / Lucas B Dillard / Stephany J Costa Dos Santos / Venkata P Dandey / Robert C Dutcher / Elizabeth G Viverette / Robert N Wine / Jennifer Woodworth / Zachary T Campbell / Marvin Wickens / Mario J Borgnia / Judith Kimble / Traci M Tanaka Hall /
PubMed AbstractPUF RNA-binding proteins are broadly conserved stem cell regulators. Nematode PUF proteins maintain germline stem cells (GSCs) and, with key partner proteins, repress differentiation mRNAs, including ...PUF RNA-binding proteins are broadly conserved stem cell regulators. Nematode PUF proteins maintain germline stem cells (GSCs) and, with key partner proteins, repress differentiation mRNAs, including gld-1. Here we report that PUF protein FBF-2 and its partner LST-1 form a ternary complex that represses gld-1 via a pair of adjacent FBF binding elements (FBEs) in its 3'UTR. One LST-1 molecule links two FBF-2 molecules via motifs in the LST-1 intrinsically-disordered region; the gld-1 FBE pair includes a well-established 'canonical' FBE and a newly-identified noncanonical FBE. Remarkably, this FBE pair drives both full RNA repression in GSCs and full RNA activation upon differentiation. Discoveries of the LST-1-FBF-2 ternary complex, the gld-1 adjacent FBEs, and their in vivo significance predict an expanded regulatory repertoire of different assemblies of PUF-partner-RNA higher order complexes in nematode GSCs. This also suggests analogous PUF controls may await discovery in other biological contexts and organisms.
External linksNat Commun / PubMed:39747099 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.2 - 6.4 Å
Structure data

EMDB-45096: The FBF-2/LST-1/gld-1 RNA complex (one FBF-2)
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-45097: The FBF-2/LST-1/gld-1 RNA complex (two FBF-2)
Method: EM (single particle) / Resolution: 6.4 Å

PDB-8viv:
Crystal structure of FBF-2 RBD in complex with gld-1 FBEa* RNA
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • caenorhabditis elegans (invertebrata)
  • Caenorhabditis elegans/Monacrosporium haptotylum mixed EST library (eukaryote)
KeywordsRNA BINDING PROTEIN / PUF protein

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