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TitleNon-neutralizing SARS-CoV-2 N-terminal domain antibodies protect mice against severe disease using Fc-mediated effector functions.
Journal, issue, pagesPLoS Pathog, Vol. 20, Issue 6, Page e1011569, Year 2024
Publish dateJun 20, 2024
AuthorsCamille N Pierre / Lily E Adams / Jaclyn S Higgins / Kara Anasti / Derrick Goodman / Dieter Mielke / Sherry Stanfield-Oakley / John M Powers / Dapeng Li / Wes Rountree / Yunfei Wang / Robert J Edwards / S Munir Alam / Guido Ferrari / Georgia D Tomaras / Barton F Haynes / Ralph S Baric / Kevin O Saunders /
PubMed AbstractAntibodies perform both neutralizing and non-neutralizing effector functions that protect against certain pathogen-induced diseases. A human antibody directed at the SARS-CoV-2 Spike N-terminal ...Antibodies perform both neutralizing and non-neutralizing effector functions that protect against certain pathogen-induced diseases. A human antibody directed at the SARS-CoV-2 Spike N-terminal domain (NTD), DH1052, was recently shown to be non-neutralizing, yet it protected mice and cynomolgus macaques from severe disease. The mechanisms of NTD non-neutralizing antibody-mediated protection are unknown. Here we show that Fc effector functions mediate NTD non-neutralizing antibody (non-nAb) protection against SARS-CoV-2 MA10 viral challenge in mice. Though non-nAb prophylactic infusion did not suppress infectious viral titers in the lung as potently as neutralizing antibody (nAb) infusion, disease markers including gross lung discoloration were similar in nAb and non-nAb groups. Fc functional knockout substitutions abolished non-nAb protection and increased viral titers in the nAb group. Fc enhancement increased non-nAb protection relative to WT, supporting a positive association between Fc functionality and degree of protection from SARS-CoV-2 infection. For therapeutic administration of antibodies, non-nAb effector functions contributed to virus suppression and lessening of lung discoloration, but the presence of neutralization was required for optimal protection from disease. This study demonstrates that non-nAbs can utilize Fc-mediated mechanisms to lower viral load and prevent lung damage due to coronavirus infection.
External linksPLoS Pathog / PubMed:38900807 / PubMed Central
MethodsEM (single particle)
Resolution15.0 Å
Structure data

EMDB-44644: SARS CoV2 spike in complex with NTD-directed antibody Fab DH1052
Method: EM (single particle) / Resolution: 15.0 Å

Source
  • Severe acute respiratory syndrome coronavirus 2
  • Homo sapiens (human)

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