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Yorodumi- EMDB-44644: SARS CoV2 spike in complex with NTD-directed antibody Fab DH1052 -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-44644 | |||||||||
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Title | SARS CoV2 spike in complex with NTD-directed antibody Fab DH1052 | |||||||||
Map data | CoV2 spike in complex with antibody Fab DH1052 | |||||||||
Sample |
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Keywords | SARS coronavirus spike antibody / VIRAL PROTEIN | |||||||||
Biological species | Severe acute respiratory syndrome coronavirus 2 / Homo sapiens (human) | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 15.0 Å | |||||||||
Authors | Edwards RJ / Mansouri K | |||||||||
Funding support | United States, 1 items
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Citation | Journal: PLoS Pathog. Title: Non-neutralizing SARS-CoV-2 N-terminal domain antibodies protects mice against severe disease using Fc effector functions Authors: Pierre CN / Adams LE / Anasti K / Goodman D / Stanfield-Oakley S / Li D / Rountree W / Wang Y / Edwards RJ / Alam SM / Ferrari G / Tomaras GD / Haynes BF / Baric RS / Saunders KO | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_44644.map.gz | 3.1 MB | EMDB map data format | |
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Header (meta data) | emd-44644-v30.xml emd-44644.xml | 18.2 KB 18.2 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_44644_fsc.xml | 3.5 KB | Display | FSC data file |
Images | emd_44644.png | 51.6 KB | ||
Filedesc metadata | emd-44644.cif.gz | 5.1 KB | ||
Others | emd_44644_half_map_1.map.gz emd_44644_half_map_2.map.gz | 2.4 MB 2.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-44644 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-44644 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_44644.map.gz / Format: CCP4 / Size: 3.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | CoV2 spike in complex with antibody Fab DH1052 | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.74 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half map 1
File | emd_44644_half_map_1.map | ||||||||||||
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Annotation | Half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 2
File | emd_44644_half_map_2.map | ||||||||||||
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Annotation | Half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Complex of SARS CoV2 spike with NTD-directed antibody Fab DH1052
Entire | Name: Complex of SARS CoV2 spike with NTD-directed antibody Fab DH1052 |
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Components |
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-Supramolecule #1: Complex of SARS CoV2 spike with NTD-directed antibody Fab DH1052
Supramolecule | Name: Complex of SARS CoV2 spike with NTD-directed antibody Fab DH1052 type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
Molecular weight | Theoretical: 372 KDa |
-Supramolecule #2: DH1052 antibody Fab
Supramolecule | Name: DH1052 antibody Fab / type: complex / ID: 2 / Parent: 1 |
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Source (natural) | Organism: Homo sapiens (human) |
-Supramolecule #3: SARS CoV2 spike
Supramolecule | Name: SARS CoV2 spike / type: complex / ID: 3 / Parent: 1 |
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Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.1 mg/mL | |||||||||
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Buffer | pH: 7.4 Component:
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Staining | Type: NEGATIVE / Material: Uranyl formate Details: A 5 microliter drop of complex was incubated on the grid for 10 to 15 s, blotted and then stained with 2 percent uranyl formate for 1 minute, then blotted and air dried. | |||||||||
Grid | Model: Homemade / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 3 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec. | |||||||||
Details | Spike protein and Fab protein at 1 mg per ml were stored at minus 80 degrees C until use. Spike was thawed in an aluminum block at 37 degrees C for 5 minutes, Fab was thawed in an aluminum block at room temperature for 5 minutes. To for the complex, 2 microliters of spike was mixed with Fab at a 1 to 9 ratio spike to Fab, and incubated at 37 degrees C for 1 hour. The complex was then crosslinked by diluting to a final spike concentration of 0.1 mg per ml into room temperature buffer containing 150 mM NaCl, 20 mM HEPES pH 7.4, 5 percent glycerol, and 7.5 mM glutaraldehyde. After 5 minutes cross-linking, excess glutaraldehyde was quenched by adding sufficient 1 M Tris pH 7.4 stock to give a final concentration of 75 mM Tris and incubated for 5 minutes. |
-Electron microscopy
Microscope | FEI/PHILIPS EM420 |
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Electron beam | Acceleration voltage: 120 kV / Electron source: LAB6 |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.0 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 82000 |
Sample stage | Specimen holder model: SIDE ENTRY, EUCENTRIC |
Image recording | Film or detector model: OTHER / Digitization - Dimensions - Width: 2048 pixel / Digitization - Dimensions - Height: 2048 pixel / Number grids imaged: 1 / Number real images: 200 / Average exposure time: 0.5 sec. / Average electron dose: 32.0 e/Å2 |
-Image processing
-Atomic model buiding 1
Initial model | PDB ID: Chain - Source name: PDB / Chain - Initial model type: experimental model |
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Refinement | Space: REAL / Protocol: RIGID BODY FIT / Target criteria: cross correlation |