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TitleThe hypertrophic cardiomyopathy-associated A331P actin variant enhances basal contractile activity and elicits resting muscle dysfunction.
Journal, issue, pagesiScience, Vol. 28, Issue 2, Page 111816, Year 2025
Publish dateFeb 21, 2025
AuthorsMatthew H Doran / Michael J Rynkiewicz / Evan Despond / Meera C Viswanathan / Aditi Madan / Kripa Chitre / Axel J Fenwick / Duncan Sousa / William Lehman / John F Dawson / Anthony Cammarato /
PubMed AbstractPrevious studies aimed at defining the mechanistic basis of hypertrophic cardiomyopathy caused by A331P cardiac actin have reported conflicting results. The mutation is located along an actin surface ...Previous studies aimed at defining the mechanistic basis of hypertrophic cardiomyopathy caused by A331P cardiac actin have reported conflicting results. The mutation is located along an actin surface strand, proximal to residues that interact with tropomyosin. These F-actin-tropomyosin associations are vital for proper contractile inhibition. To help resolve disease pathogenesis, we implemented a multidisciplinary approach. Transgenic , expressing A331P actin, displayed skeletal muscle hypercontraction and elevated basal myocardial activity. A331P thin filaments, reconstituted using recombinant human cardiac actin, exhibited higher myosin-based sliding speeds, exclusively at low Ca concentrations. Cryo-EM-based reconstructions revealed no detectable A331P-related structural perturbations in F-actin. , however, the P331-containing actin surface strand was less mobile and established diminished van der Waal's attractive forces with tropomyosin, which correlated with greater variability in inhibitory tropomyosin positioning. Such mutation-induced effects potentially elevate resting contractile activity among our models and may stimulate pathology in patients.
External linksiScience / PubMed:39981516 / PubMed Central
MethodsEM (helical sym.)
Resolution3.5 Å
Structure data

44154

9b3r

EMDB-44154, PDB-9b3r:
The structure of human cardiac F-actin
Method: EM (helical sym.) / Resolution: 3.5 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • Spodoptera frugiperda (fall armyworm)
  • homo sapiens (human)
KeywordsCONTRACTILE PROTEIN / actin / cardiac / human / sarcomere

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