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TitleThe nucleocapsid architecture and structural atlas of the prototype baculovirus define the hallmarks of a new viral realm.
Journal, issue, pagesSci Adv, Vol. 10, Issue 51, Page eado2631, Year 2024
Publish dateDec 20, 2024
AuthorsBronte A Johnstone / Joshua M Hardy / Jungmin Ha / Anamarija Butkovic / Paulina Koszalka / Cathy Accurso / Hariprasad Venugopal / Alex de Marco / Mart Krupovic / Fasséli Coulibaly /
PubMed AbstractBaculovirus is the most studied insect virus owing to a broad ecological distribution and ease of engineering for biotechnological applications. However, its structure and evolutionary place in the ...Baculovirus is the most studied insect virus owing to a broad ecological distribution and ease of engineering for biotechnological applications. However, its structure and evolutionary place in the virosphere remain enigmatic. Using cryo-electron microscopy, we show that the nucleocapsid forms a covalently cross-linked helical tube protecting a highly compacted 134-kilobase pair DNA genome. The ends of the tube are sealed by the base and cap substructures, which share a 126-subunit hub but differ in components that promote actin tail-mediated propulsion and nuclear entry of the nucleocapsid, respectively. Unexpectedly, sensitive searches for hidden evolutionary links show that the morphogenetic machinery and conserved oral infectivity factors originated within the lineage of baculo-like viruses (class ). The unique viral architecture and structural atlas of hallmark proteins firmly place these viruses into a separate new realm, the highest taxonomy rank, and provide a structural framework to expand their use as sustainable bioinsecticides and biomedical tools.
External linksSci Adv / PubMed:39693434 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution3.06 - 6.3 Å
Structure data

EMDB-43585: Structure of the AcMNPV baculovirus VP39 nucleocapsid (C14 helical reconstruction)
Method: EM (helical sym.) / Resolution: 4.24 Å

43586

8vwh

EMDB-43586, PDB-8vwh:
Structure of the baculovirus major nucleocapsid protein VP39 (localised reconstruction)
Method: EM (single particle) / Resolution: 3.06 Å

EMDB-43587: The base complex of the baculovirus nucleocapsid (Autographa californica multiple nucleopolyhedrovirus)
Method: EM (single particle) / Resolution: 4.98 Å

43589

8vwj

EMDB-43589, PDB-8vwj:
The base complex of the AcMNPV baculovirus nucleocapsid (Class 2, localised reconstruction)
Method: EM (single particle) / Resolution: 4.78 Å

EMDB-43590: The cap complex of the baculovirus nucleocapsid (Autographa californica multiple nucleopolyhedrovirus)
Method: EM (single particle) / Resolution: 6.3 Å

EMDB-43591: The cap of the AcMNPV baculovirus nucleocapsid (localised reconstruction)
Method: EM (single particle) / Resolution: 5.05 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • autographa californica multiple nucleopolyhedrovirus
KeywordsVIRAL PROTEIN / Capsid / helical assembly / Nucleocapsid / Base / baculovirus / virus / AcMNPV / VP39

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