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TitleVisualizing late states of human 40S ribosomal subunit maturation.
Journal, issue, pagesNature, Vol. 558, Issue 7709, Page 249-253, Year 2018
Publish dateJun 6, 2018
AuthorsMichael Ameismeier / Jingdong Cheng / Otto Berninghausen / Roland Beckmann /
PubMed AbstractThe formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high- ...The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3' rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage.
External linksNature / PubMed:29875412
MethodsEM (single particle)
Resolution3.5 - 4.5 Å
Structure data

EMDB-4337, PDB-6g18:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State C
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-4348, PDB-6g4s:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State B
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-4349, PDB-6g4w:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State A
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-4350, PDB-6g51:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State D
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-4351, PDB-6g53:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State E
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-4352, PDB-6g5h:
Cryo-EM structure of a late human pre-40S ribosomal subunit - Mature
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-4353, PDB-6g5i:
Cryo-EM structure of a late human pre-40S ribosomal subunit - State R
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRIBOSOME / 40S / pre-40S / ribosome biogenesis

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