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TitleStructural Basis for NusA Stabilized Transcriptional Pausing.
Journal, issue, pagesMol Cell, Vol. 69, Issue 5, Page 816-827.e4, Year 2018
Publish dateMar 1, 2018
AuthorsXieyang Guo / Alexander G Myasnikov / James Chen / Corinne Crucifix / Gabor Papai / Maria Takacs / Patrick Schultz / Albert Weixlbaumer /
PubMed AbstractTranscriptional pausing by RNA polymerases (RNAPs) is a key mechanism to regulate gene expression in all kingdoms of life and is a prerequisite for transcription termination. The essential bacterial ...Transcriptional pausing by RNA polymerases (RNAPs) is a key mechanism to regulate gene expression in all kingdoms of life and is a prerequisite for transcription termination. The essential bacterial transcription factor NusA stimulates both pausing and termination of transcription, thus playing a central role. Here, we report single-particle electron cryo-microscopy reconstructions of NusA bound to paused E. coli RNAP elongation complexes with and without a pause-enhancing hairpin in the RNA exit channel. The structures reveal four interactions between NusA and RNAP that suggest how NusA stimulates RNA folding, pausing, and termination. An asymmetric translocation intermediate of RNA and DNA converts the active site of the enzyme into an inactive state, providing a structural explanation for the inhibition of catalysis. Comparing RNAP at different stages of pausing provides insights on the dynamic nature of the process and the role of NusA as a regulatory factor.
External linksMol Cell / PubMed:29499136 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 4.1 Å
Structure data

4274

6flp

EMDB-4274, PDB-6flp:
CryoEM structure of E.coli RNA polymerase paused elongation complex without RNA hairpin bound to NusA
Method: EM (single particle) / Resolution: 4.1 Å

4275

6flq

EMDB-4275, PDB-6flq:
CryoEM structure of E.coli RNA polymerase paused elongation complex bound to NusA
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • Escherichia coli K-12 (bacteria)
  • synthetic construct (others)
  • escherichia coli (strain k12) (bacteria)
KeywordsTRANSCRIPTION / RNA polymerase / Transcriptional pausing / his pause / NusA

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