+Search query
-Structure paper
Title | Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit. |
---|---|
Journal, issue, pages | EMBO J, Vol. 37, Issue 7, Year 2018 |
Publish date | Apr 3, 2018 |
Authors | Alain Scaiola / Cohue Peña / Melanie Weisser / Daniel Böhringer / Marc Leibundgut / Purnima Klingauf-Nerurkar / Stefan Gerhardy / Vikram Govind Panse / Nenad Ban / |
PubMed Abstract | Final maturation of eukaryotic ribosomes occurs in the cytoplasm and requires the sequential removal of associated assembly factors and processing of the immature 20S pre-RNA Using cryo-electron ...Final maturation of eukaryotic ribosomes occurs in the cytoplasm and requires the sequential removal of associated assembly factors and processing of the immature 20S pre-RNA Using cryo-electron microscopy (cryo-EM), we have determined the structure of a yeast cytoplasmic pre-40S particle in complex with Enp1, Ltv1, Rio2, Tsr1, and Pno1 assembly factors poised to initiate final maturation. The structure reveals that the pre-rRNA adopts a highly distorted conformation of its 3' major and 3' minor domains stabilized by the binding of the assembly factors. This observation is consistent with a mechanism that involves concerted release of the assembly factors orchestrated by the folding of the rRNA in the head of the pre-40S subunit during the final stages of maturation. Our results provide a structural framework for the coordination of the final maturation events that drive a pre-40S particle toward the mature form capable of engaging in translation. |
External links | EMBO J / PubMed:29459436 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.1 - 4.3 Å |
Structure data | EMDB-4214, PDB-6fai: EMDB-4215: EMDB-4216: EMDB-4217: EMDB-4218: |
Chemicals | ChemComp-ZN: ChemComp-MG: |
Source |
|
Keywords | RIBOSOME / pre-40S ribosme / cryo-EM / ribosome assembly / ribosomes / ribosome biogenesis |