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TitleMulti-species cryoEM calibration and workflow verification standard.
Journal, issue, pagesActa Crystallogr F Struct Biol Commun, Vol. 80, Issue Pt 11, Page 320-327, Year 2024
Publish dateNov 1, 2024
AuthorsDaija Bobe / Mykhailo Kopylov / Jessalyn Miller / Aaron P Owji / Edward T Eng /
PubMed AbstractCryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established ...Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. Here, a cryoEM calibration sample consisting of a mixture of compatible macromolecules is introduced that can not only be used for resolution optimization, but also provides multiple reference points for evaluating instrument performance, data quality and image-processing workflows in a single experiment. This combined test specimen provides researchers with a reference point for validating their cryoEM pipeline, benchmarking their methodologies and testing new algorithms.
External linksActa Crystallogr F Struct Biol Commun / PubMed:39494502 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution2.38 - 3.36 Å
Structure data

EMDB-41917: CryoEM structure of PP7 virus-like particle from a multi-species dataset
Method: EM (single particle) / Resolution: 3.36 Å

EMDB-41919: Structure of E.coli beta-Galactosidase from a multi-species dataset
Method: EM (single particle) / Resolution: 2.73 Å

EMDB-41923: Structure of human Apoferritin from a multi-species dataset
Method: EM (single particle) / Resolution: 2.38 Å

EMDB-41924: Structure of Tobacco Mosaic Virus from a multi-species dataset
Method: EM (helical sym.) / Resolution: 2.4 Å

Source
  • Escherichia coli (E. coli)
  • Homo sapiens (human)

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