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| タイトル | Multi-species cryoEM calibration and workflow verification standard. |
|---|---|
| ジャーナル・号・ページ | Acta Crystallogr F Struct Biol Commun, Vol. 80, Issue Pt 11, Page 320-327, Year 2024 |
| 掲載日 | 2024年11月1日 |
著者 | Daija Bobe / Mykhailo Kopylov / Jessalyn Miller / Aaron P Owji / Edward T Eng / ![]() |
| PubMed 要旨 | Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established ...Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. Here, a cryoEM calibration sample consisting of a mixture of compatible macromolecules is introduced that can not only be used for resolution optimization, but also provides multiple reference points for evaluating instrument performance, data quality and image-processing workflows in a single experiment. This combined test specimen provides researchers with a reference point for validating their cryoEM pipeline, benchmarking their methodologies and testing new algorithms. |
リンク | Acta Crystallogr F Struct Biol Commun / PubMed:39494502 / PubMed Central |
| 手法 | EM (単粒子) / EM (らせん対称) |
| 解像度 | 2.38 - 3.36 Å |
| 構造データ | ![]() EMDB-41917: CryoEM structure of PP7 virus-like particle from a multi-species dataset ![]() EMDB-41919: Structure of E.coli beta-Galactosidase from a multi-species dataset ![]() EMDB-41923: Structure of human Apoferritin from a multi-species dataset ![]() EMDB-41924: Structure of Tobacco Mosaic Virus from a multi-species dataset |
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Homo sapiens (ヒト)