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TitleCryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes.
Journal, issue, pagesJ Cell Biol, Vol. 222, Issue 12, Year 2023
Publish dateDec 4, 2023
AuthorsYue Wang / Jun Yang / Fangheng Hu / Yuchen Yang / Kaiyao Huang / Kai Zhang /
PubMed AbstractMastigonemes are thread-like structures adorning the flagella of protists. In Chlamydomonas reinhardtii, filamentous mastigonemes find their roots in the flagella's distal region, associated with the ...Mastigonemes are thread-like structures adorning the flagella of protists. In Chlamydomonas reinhardtii, filamentous mastigonemes find their roots in the flagella's distal region, associated with the channel protein PKD2, implying their potential contribution to external signal sensing and flagellar motility control. Here, we present the single-particle cryo-electron microscopy structure of the mastigoneme at 3.4 Å. The filament unit, MST1, consists of nine immunoglobulin-like domains and six Sushi domains, trailed by an elastic polyproline-II helix. Our structure demonstrates that MST1 subunits are periodically assembled to form a centrosymmetric, non-polar filament. Intriguingly, numerous clustered disulfide bonds within a ladder-like spiral configuration underscore structural resilience. While defects in the mastigoneme structure did not noticeably affect general attributes of cell swimming, they did impact specific swimming properties, particularly under varied environmental conditions such as redox shifts and heightened viscosity. Our findings illuminate the potential role of mastigonemes in flagellar motility and suggest their involvement in diverse environmental responses.
External linksJ Cell Biol / PubMed:37882754 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 3.9 Å
Structure data

41674

8tx1

EMDB-41674, PDB-8tx1:
Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Structure at 3.6A
Method: EM (single particle) / Resolution: 3.62 Å

41679

8txb

EMDB-41679, PDB-8txb:
Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Structure at 3.9A
Method: EM (single particle) / Resolution: 3.9 Å

41680

8txc

EMDB-41680, PDB-8txc:
Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Subunit MST1 Structure at 3.9 angstrom
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-41696: Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Structure at 3.4A
Method: EM (single particle) / Resolution: 3.4 Å

Source
  • chlamydomonas reinhardtii (plant)
KeywordsSTRUCTURAL PROTEIN / Hair-like structures attached to the protistan flagella / Fibrous Flagellar Hairs

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