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TitleStructural and quantum chemical basis for OCP-mediated quenching of phycobilisomes.
Journal, issue, pagesSci Adv, Vol. 10, Issue 14, Page eadk7535, Year 2024
Publish dateApr 5, 2024
AuthorsPaul V Sauer / Lorenzo Cupellini / Markus Sutter / Mattia Bondanza / María Agustina Domínguez Martin / Henning Kirst / David Bína / Adrian Fujiet Koh / Abhay Kotecha / Basil J Greber / Eva Nogales / Tomáš Polívka / Benedetta Mennucci / Cheryl A Kerfeld /
PubMed AbstractCyanobacteria use large antenna complexes called phycobilisomes (PBSs) for light harvesting. However, intense light triggers non-photochemical quenching, where the orange carotenoid protein (OCP) ...Cyanobacteria use large antenna complexes called phycobilisomes (PBSs) for light harvesting. However, intense light triggers non-photochemical quenching, where the orange carotenoid protein (OCP) binds to PBS, dissipating excess energy as heat. The mechanism of efficiently transferring energy from phycocyanobilins in PBS to canthaxanthin in OCP remains insufficiently understood. Using cryo-electron microscopy, we unveiled the OCP-PBS complex structure at 1.6- to 2.1-angstrom resolution, showcasing its inherent flexibility. Using multiscale quantum chemistry, we disclosed the quenching mechanism. Identifying key protein residues, we clarified how canthaxanthin's transition dipole moment in its lowest-energy dark state becomes large enough for efficient energy transfer from phycocyanobilins. Our energy transfer model offers a detailed understanding of the atomic determinants of light harvesting regulation and antenna architecture in cyanobacteria.
External linksSci Adv / PubMed:38578996 / PubMed Central
MethodsEM (single particle)
Resolution1.63 - 2.2 Å
Structure data

41434

8to2

EMDB-41434, PDB-8to2:
Bottom cylinder of high-resolution phycobilisome quenched by OCP (local refinement)
Method: EM (single particle) / Resolution: 2.0 Å

41435

8to5

EMDB-41435, PDB-8to5:
Central rod disk in C1 symmetry of high-resolution phycobilisome quenched by OCP (local refinement)
Method: EM (single particle) / Resolution: 1.87 Å

EMDB-41436: Central rod disk in D3 symmetry of high-resolution phycobilisome quenched by OCP (local refinement)
Method: EM (single particle) / Resolution: 1.63 Å

EMDB-41463: Synechocystis PCC 6803 Phycobilisome quenched by OCP, high resolution
Method: EM (single particle) / Resolution: 2.2 Å

41475

8tpj

EMDB-41475, PDB-8tpj:
Top cylinder bound to OCP from high-resolution phycobilisome quenched by OCP (local refinement)
Method: EM (single particle) / Resolution: 2.1 Å

41585

8tro

EMDB-41585, PDB-8tro:
Rod from high-resolution phycobilisome quenched by OCP (local refinement)
Method: EM (single particle) / Resolution: 1.9 Å

Chemicals

ChemComp-CYC:
PHYCOCYANOBILIN / Phycocyanobilin

ChemComp-45D:
beta,beta-carotene-4,4'-dione / Canthaxanthin

ChemComp-HOH:
WATER / Water

Source
  • Synechocystis sp. (strain PCC 6803 / Kazusa) (bacteria)
  • synechocystis sp. pcc 6803 (bacteria)
KeywordsPHOTOSYNTHESIS / Complex / light harvesting / pigment

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