Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of human 20S proteasome biogenesis.
Journal, issue, pages	Nat Commun, Vol. 15, Issue 1, Page 8184, Year 2024
Publish date	Sep 18, 2024
Authors	Hanxiao Zhang / Chenyu Zhou / Zarith Mohammad / Jianhua Zhao /
PubMed Abstract	New proteasomes are produced to accommodate increases in cellular catabolic demand and prevent the accumulation of cytotoxic proteins. Formation of the proteasomal 20S core complex relies on the ...New proteasomes are produced to accommodate increases in cellular catabolic demand and prevent the accumulation of cytotoxic proteins. Formation of the proteasomal 20S core complex relies on the function of the five chaperones PAC1-4 and POMP. Here, to understand how these chaperones facilitate proteasome assembly, we tagged the endogenous chaperones using CRISPR/Cas gene editing and examined the chaperone-bound complexes by cryo-EM. We observe an early α-ring intermediate subcomplex that is stabilized by PAC1-4, which transitions to β-ring assembly upon dissociation of PAC3/PAC4 and rearrangement of the PAC1 N-terminal tail. Completion of the β-ring and dimerization of half-proteasomes repositions critical lysine K33 to trigger cleavage of the β pro-peptides, leading to the concerted dissociation of POMP and PAC1/PAC2 to yield mature 20S proteasomes. This study reveals structural insights into critical points along the assembly pathway of the human proteasome and provides a molecular blueprint for 20S biogenesis.
External links	Nat Commun / PubMed:39294158 / PubMed Central
Methods	EM (single particle)
Resolution	2.8 - 3.0 Å
Structure data	41377 8tm3 EMDB-41377, PDB-8tm3: Human proteasome alpha ring assembly intermediate Method: EM (single particle) / Resolution: 3.0 Å 41378 8tm4 EMDB-41378, PDB-8tm4: Human pre 13S proteasome assembly intermediate Method: EM (single particle) / Resolution: 3.0 Å 41379 8tm5 EMDB-41379, PDB-8tm5: Human mixed 13S proteasome assembly intermediate Method: EM (single particle) / Resolution: 3.0 Å 41380 8tm6 EMDB-41380, PDB-8tm6: Human premature 20S proteasome assembly intermediate Method: EM (single particle) / Resolution: 2.8 Å EMDB-41381: Premature human 20S proteasome assembly intermediate 37C Method: EM (single particle) / Resolution: 2.8 Å
Source	homo sapiens (human)
Keywords	HYDROLASE / proteasome / proteasome assembly / PAC3 / PAC4 / POMP / beta ring assembly / 20S / proteasome core particle